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PMID: 338584 Published · ppublish English Journal Article

Effect of thymine concentration on cell shape in Thy- Escherichia coli B/r.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 320-8

Meacock PA, Pritchard RH, Roberts EM

Abstract

Cells of a thymineless mutant of Escherichia coli B/r are shown to change their shape when the concentration of thymine in the growth medium is reduced. Electron micrographs of whole cells and isolated sacculi were used to make quantitative measurements of the changes in cell length and width which occur as a result of such a change in thymine concentration. The results showed that there is an increase in cell volume, which is due to an increase in cell width accompanied by a decrease in cell length. These changes were compared with the predictions of models which assume that cell shape is influenced by the chromosome replication cycle.

MeSH Terms
DNA, Bacterial/metabolism Escherichia coli/cytology,drug effects Microscopy, Electron Models, Biological Thymine/metabolism,pharmacology
Chemicals
DNA, Bacterial Thymine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meacock P A
Pritchard R H
Roberts E M
References (25)
25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.1.320-328.1978
Published
1978-01-00
Pages
320-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222010
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.1.320-328.1978 PMC Full Text View journal details
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