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PMID: 3384472 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Developmental-form-specific DNA-binding proteins in Chlamydia spp.

Infection and immunity ·Vol. 56 ·No. 7 ·1988-07-00 ·Pages 1678-84

Wagar EA, Stephens RS

Abstract

We identified DNA-binding proteins specific to the elementary body (EB) developmental form of Chlamydia spp. Chlamydial proteins from whole lysates were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with nick-translated chlamydial DNA. By this method, C. trachomatis serovar L2 EBs had three unique protein bands of 58,000, 25,700, and 17,000 molecular weight not seen in the reticulate bodies. The 17,000-molecular-weight protein and the 25,700-molecular-weight protein were identified in an acid-soluble protein fraction and were resistant to high-salt elution, similar to other procaryotic nucleoproteins. The 17,000-molecular-weight protein was also detected in preparations with isolated chromosomes from EBs. Preliminary characterization indicated that the protein-DNA interaction was principally charge related.

MeSH Terms
Bacterial Proteins/genetics,isolation & purification Chemical Phenomena Chemistry, Physical Chlamydia/genetics,growth & development,metabolism Chlamydia trachomatis/genetics,growth & development,metabolism Chlamydophila psittaci/genetics,growth & development,metabolism Chromosomes/analysis,metabolism DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/genetics,isolation & purification Molecular Weight Radioligand Assay Species Specificity
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wagar E A
Department of Laboratory Medicine, University of California, San Francisco 94143-0412.
Stephens R S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.7.1678-1684.1988
Published
1988-07-00
Pages
1678-84
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259462
Subset
IM
Grants
NIAID NIH HHS · AI07234 · United States
External Links
PubMed source DOI: 10.1128/iai.56.7.1678-1684.1988 PMC Full Text View journal details
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