Abstract
Our study aimed to investigate the function of GALNT2 in lung adenocarcinoma (LUAD). We used network tools and tissue microarray immunohistochemistry to measure the expression levels of GALNT2 in LUAD. Kaplan-Meier curves and Cox regression methods were used in survival analysis. We detected the role of GALNT2 in cell lines by Cell Counting Kit-8, colony formation, transwell, and wound healing assays. We performed Western blotting to evaluate downstream protein levels. GALNT2 was highly expressed in LUAD samples and indicated a poor prognosis. Knockdown of GALNT2 suppressed cell line proliferation, migration, and invasion abilities, while overexpression of GALNT2 enhanced those phenotypes. Moreover, GALNT2 activated Notch/Hes1-PTEN-PI3K/Akt signaling axis. Our data confirmed the cancer-promoting effect of GALNT2, and might provide a new approach for LUAD therapy.
Keywords
GALNT2
Lung adenocarcinoma
Metastasis
Notch
Proliferation
MeSH Terms
Adenocarcinoma of Lung/genetics,metabolism,pathology
Aged
Apoptosis
Biomarkers, Tumor/genetics,metabolism
Cell Movement
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms/genetics,metabolism,pathology
Male
N-Acetylgalactosaminyltransferases/genetics,metabolism
Neoplasm Invasiveness
PTEN Phosphohydrolase/genetics,metabolism
Phosphatidylinositol 3-Kinases/genetics,metabolism
Prognosis
Proto-Oncogene Proteins c-akt/genetics,metabolism
Receptor, Notch1/genetics,metabolism
Survival Rate
Transcription Factor HES-1/genetics,metabolism
Tumor Cells, Cultured
Chemicals
Biomarkers, Tumor
NOTCH1 protein, human
Receptor, Notch1
Transcription Factor HES-1
HES1 protein, human
N-Acetylgalactosaminyltransferases
polypeptide N-acetylgalactosaminyltransferase
Proto-Oncogene Proteins c-akt
PTEN Phosphohydrolase
PTEN protein, human
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Wang Wei
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Sun Ruiying
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Zeng Lizhong
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Chen Yang
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Zhang Na
Department of Pathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Cao Shiguang
Department of Nuclear Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Deng Shanshan
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Meng Xia
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China; Department of Pathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. Electronic address: mengxiamail@163.com.
Yang Shuanying
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. Electronic address: yangshuanying66@163.com.
