IgG anti-hapten antibody secretion in vitro commences after extensive precursor proliferation.

Immunology ·Vol. 33 ·No. 4 ·1977-10-00 ·Pages 485-90

Kemshead JT, North JR, Askonas BA

Abstract

We have investigated whether the intermediate cells that arise in primed mice after boosting require further cycles of division in culture before maturation into IgG antibody secreting cells. Killing of dividing cells between days 1--4 in culture, by exposure to BUdR-uv irradiation ablated the high IgG response observed on day 5 in control cultures. After T cell removal and replacement by a soluble factor (TRF) similar results were obtained. Thus B cell division over an extended period occurs prior to the appearance of IgG secreting cells. Furthermore, autoradiography of plaques from cultures briefly exposed to [3H]thymidine before harvest showed that some antibody secreting cells were synthesizing DNA at the time of assay.

MeSH Terms

Animals Antibody-Producing Cells B-Lymphocytes/immunology Bromodeoxyuridine/pharmacology Cell Division/drug effects DNA/biosynthesis Haptens Hemolytic Plaque Technique Immunoglobulin G In Vitro Techniques Mice Spleen/immunology

Chemicals

Haptens Immunoglobulin G DNA Bromodeoxyuridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kemshead J T

North J R

Askonas B A

References (9)

9 references, click to expand

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Article Info

Journal

Immunology

Abbr.

Immunology

ISSN

0019-2805

Published

1977-10-00

Pages

485-90

Language

English

Region

England

NLM ID

0374672

PMCID

PMC1445380

Subset

External Links

PubMed source PMC Full Text View journal details

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IgG anti-hapten antibody secretion in vitro commences after extensive precursor proliferation.

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