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PMID: 3360749 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Abundant expression of Pseudomonas genes for chlorocatechol metabolism.

Journal of bacteriology ·Vol. 170 ·No. 5 ·1988-05-00 ·Pages 2412-3

Ngai KL, Ornston LN

Abstract

The respective specific activities of catechol 1,2-oxygenase II (catechol 1,2-dioxygenase; EC 1.13.11.1) and muconate cycloisomerase II (chloromuconate cycloisomerase; EC 5.5.1.7) in crude extracts of chlorobenzoate-grown Pseudomonas cells corresponded to about 16 and 11% of the soluble cell protein. High levels of protein synthesis appeared to compensate for a loss in catalytic activity that accompanied evolutionary acquisition of broad substrate specificity required for the enzymes to accommodate halogenated substrates.

MeSH Terms

Amino Acid Sequence Base Sequence Catechol 1,2-Dioxygenase Catechols/metabolism Chemical Phenomena Chemistry Dioxygenases Gene Expression Regulation Genes, Bacterial Intramolecular Lyases Isomerases/genetics,metabolism Oxygenases/genetics,metabolism Pseudomonas/enzymology,genetics,metabolism Substrate Specificity

Chemicals

Catechols Oxygenases Dioxygenases Catechol 1,2-Dioxygenase Isomerases Intramolecular Lyases chloromuconate cycloisomerase 4-chlorocatechol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ngai K L

Department of Biology, Yale University, New Haven, Connecticut 06511.

Ornston L N

References (12)

12 references, click to expand

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Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation.
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J Biol Chem. 1966 Aug 25;241(16):3795-9 PMID: 5330966
Relationships among enzymes of the beta-ketoadipate pathway. I. Properties of cis,cis-muconate-lactonizing enzyme and muconolactone isomerase from Pseudomonas putida.
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Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
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Chemical structure and biodegradability of halogenated aromatic compounds. Two catechol 1,2-dioxygenases from a 3-chlorobenzoate-grown pseudomonad.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.170.5.2412-2413.1988

Published

1988-05-00

Pages

2412-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211142

Subset

Grants

NIGMS NIH HHS · GM-33377 · United States

External Links

PubMed source DOI: 10.1128/jb.170.5.2412-2413.1988 PMC Full Text View journal details

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Abundant expression of Pseudomonas genes for chlorocatechol metabolism.

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