Induction of intercellular communications in epithelial cell cultures.
作者:
Sharovskaja YuYu,Chailakhjan(L M),Margolis(L B)
状态:
发布时间1988-06-09
, 更新时间 2003-11-14
期刊:
Exp Cell Res
摘要:
A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxically it is the standard fluorescent microscopy itself (that is, blue light of 320- to 480-nm wavelength) which induces rapid morphological alterations of cell culture followed by the transfer of fluorescent dye from one cell to another. Thus monitoring cell-cell dye coupling by fluorescent microscopy may itself induce the dye coupling in previously uncoupled epithelial cells.