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PMID: 3357758 Published · ppublish English Journal Article

A low cost high intensity flash device for photolysis experiments.

Pflugers Archiv : European journal of physiology ·Vol. 411 ·No. 2 ·1988-02-00 ·Pages 200-3

Rapp G, Güth K

Abstract

Novel techniques of flash photolysis experiments require high intensity light sources in the near UV. We describe here a simple and inexpensive flash device which may complete with bulky and expensive laser systems if the experiments do not necessitate very short light pulses. Using a particular optical arrangement and stored electrical energy, a variation of the parameters voltage and capacitance led to a difference in light output by a factor of more than two. The system is used to relax both skeletal and smooth muscle fibres in the rigor state by releasing up to 2 mM ATP from 12.5 mM caged-ATP.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Muscle Contraction Muscle Relaxation Muscle, Smooth/physiology Muscles/physiology Photolysis Technology
Chemicals
Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rapp G
Abteilung Biophysik, Max-Planck-Institut für medizinische Forschung, Heidelberg, Federal Republic of Germany.
Güth K
References (5)
5 references, click to expand
  1. Time-Resolved Structural Studies on Insect Flight Muscle after Photolysis of Caged-ATP.
    Biophys J. 1986 Nov;50(5):993-7 PMID: 19431691
  2. Rapid photolytic release of adenosine 5'-triphosphate from a protected analogue: utilization by the Na:K pump of human red blood cell ghosts.
    Biochemistry. 1978 May 16;17(10):1929-35 PMID: 148906
  3. A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7237-41 PMID: 6938971
  4. Relaxation of rabbit psoas muscle fibres from rigor by photochemical generation of adenosine-5'-triphosphate.
    J Physiol. 1984 Sep;354:577-604 PMID: 6481645
  5. Physiological and pharmacological manipulations with light flashes.
    Annu Rev Biophys Bioeng. 1982;11:151-75 PMID: 7049061
Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
Published
1988-02-00
Pages
200-3
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
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