Home LiteratureArticle Details
PMID: 3355532 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hyaluronic acid in human articular cartilage. Age-related changes in content and size.

The Biochemical journal ·Vol. 250 ·No. 2 ·1988-03-01 ·Pages 435-41

Holmes MW, Bayliss MT, Muir H

Abstract

Total tissue content and molecular mass of hyaluronic acid was determined in papain digests of human articular cartilage using a sensitive radiosorbent assay [Laurent & Tengblad (1980) Anal. Biochem. 109, 386-394]. 1) Hyaluronic acid content increased from 0.5 microgram/mg wet wt. to 2.5 micrograms/mg wet wt. between the ages of 2.5 years and 86 years. 2) Hyaluronic acid chain size decreased from Mr 2.0 x 10(6) to 3.0 x 10(5) over the same age range. 3) There was no age-related change in the size of newly-synthesized hyaluronic acid, which was of very high molecular mass, in both immature and mature cartilage. The results are consistent with an age-related decrease in proteoglycan aggregate size and suggest that modification of the hyaluronic acid chain may take place in the extracellular matrix.

MeSH Terms
Adolescent Adult Aged Aged, 80 and over Aging/metabolism Cartilage, Articular/metabolism Child Child, Preschool Chromatography, Gel Humans Hyaluronic Acid/metabolism Hydroxyproline/analysis Macromolecular Substances Middle Aged Molecular Weight Radioimmunoassay Uronic Acids/analysis
Chemicals
Macromolecular Substances Uronic Acids Hyaluronic Acid Hydroxyproline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Holmes M W
Kennedy Institute of Rheumatology, Division of Biochemistry, Hammersmith, London, U.K.
Bayliss M T
Muir H
References (40)
40 references, click to expand
  1. Extraction, fractionation and characterization of proteoglycans from bovine tracheal cartilage.
    Biochim Biophys Acta. 1972 Nov 28;285(1):181-92 PMID: 4265839
  2. A modified uronic acid carbazole reaction.
    Anal Biochem. 1962 Oct;4:330-4 PMID: 13971270
  3. Free radicals and inflammation: protection of synovial fluid by superoxide dismutase.
    Science. 1974 Aug 9;185(4150):529-31 PMID: 4841157
  4. Aggregation of cartilage proteoglycans. 3. Characteristics of the proteins isolated from trypsin digests of aggregates.
    J Biol Chem. 1974 Jul 10;249(13):4250-6 PMID: 4277353
  5. The effects of proteolytic enzymes on the mechanical properties of adult human articular cartilage.
    Biochim Biophys Acta. 1976 May 28;428(3):741-60 PMID: 1276179
  6. Cartilage proteoglycans. Structure and heterogeneity of the protein core and the effects of specific protein modifications on the binding to hyaluronate.
    Biochem J. 1976 Jul 1;157(1):127-43 PMID: 134698
  7. Interaction of cartilage proteoglycans with hyaluronic acid.
    J Supramol Struct. 1977;7(1):101-20 PMID: 342830
  8. Variation of chemical composition with age in human femoral head cartilage.
    Ann Rheum Dis. 1978 Apr;37(2):168-74 PMID: 148244
  9. Hyaluronate in articular cartilage: age-related changes.
    Calcif Tissue Res. 1978 Nov 10;26(1):19-21 PMID: 737548
  10. Age-related changes in the composition and structure of human articular-cartilage proteoglycans.
    Biochem J. 1978 Dec 15;176(3):683-93 PMID: 747646
  11. The role of link-protein in the structure of cartilage proteoglycan aggregates.
    Biochem J. 1979 Jan 1;177(1):237-47 PMID: 34388
  12. Proteoglycans from bovine nasal cartilage. Properties of a soluble form of link protein.
    J Biol Chem. 1979 Oct 25;254(20):10523-31 PMID: 114526
  13. Changes with age in the glycosaminoglycans of human articular cartilage.
    Ann Rheum Dis. 1979 Aug;38(4):371-7 PMID: 496451
  14. Age-related changes in the structure of the proteoglycan subunits from human articular cartilage.
    J Biol Chem. 1980 Jan 10;255(1):217-24 PMID: 7350154
  15. Swelling pressures of proteoglycans at the concentrations found in cartilaginous tissues.
    Biorheology. 1979;16(6):447-64 PMID: 534768
  16. Effect of oxygen-derived free radicals on hyaluronic acid.
    Arthritis Rheum. 1980 Apr;23(4):455-63 PMID: 6245661
  17. Separate effects of exogenous hyaluronic acid on proteoglycan synthesis and deposition in pericellular matrix by cultured chick embryo limb chondrocytes.
    Dev Biol. 1980 Mar;75(1):121-9 PMID: 7371989
  18. Quantitative analysis of hyaluronate in nanogram amounts.
    Biochem J. 1980 Jan 1;185(1):101-5 PMID: 6445729
  19. Determination of hyaluronate in biological samples by a specific radioassay technique.
    Anal Biochem. 1980 Dec;109(2):386-94 PMID: 7224163
  20. The role of superoxide and hydroxyl radicals in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid.
    J Inorg Biochem. 1981 Apr;14(2):127-34 PMID: 6265596
  21. Plasma clearance, tissue distribution and metabolism of hyaluronic acid injected intravenously in the rabbit.
    Biochem J. 1981 Nov 15;200(2):415-24 PMID: 7340841
  22. Structure of proteoglycans from different layers of human articular cartilage.
    Biochem J. 1983 Feb 1;209(2):387-400 PMID: 6405736
  23. Effect of oxygen-derived reactive species on cartilage proteoglycan-hyaluronate aggregates.
    Biochem Int. 1984 May;8(5):629-37 PMID: 6548142
  24. Structure and interactions of cartilage proteoglycan binding region and link protein.
    Biochem J. 1985 May 15;228(1):77-85 PMID: 4004817
  25. Depolymerisation products of hyaluronic acid after exposure to oxygen-derived free radicals.
    Ann Rheum Dis. 1985 Nov;44(11):780-9 PMID: 4062391
  26. Identification of a hyaluronic acid-binding protein that interferes with the preparation of high-buoyant-density proteoglycan aggregates from adult human articular cartilage.
    Biochem J. 1985 Oct 1;231(1):129-38 PMID: 4062883
  27. Proteoglycan synthesis in suspension cultures of Swarm rat chondrosarcoma chondrocytes and inhibition by exogenous hyaluronate.
    Arch Biochem Biophys. 1986 May 1;246(2):602-10 PMID: 2423031
  28. Calibration of the relative molecular mass of proteoglycan subunit by column chromatography on Sepharose CL-2B.
    Biochem J. 1986 Apr 15;235(2):553-7 PMID: 3741405
  29. Determination of hydroxyproline.
    Clin Chim Acta. 1967 Nov;18(2):267-73 PMID: 4864804
  30. Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.
    J Biol Chem. 1969 May 10;244(9):2384-96 PMID: 5783840
  31. The determination of collagen and elastin.
    Methods Biochem Anal. 1967;15:25-76 PMID: 4899619
  32. Ionic polysaccharides. 3. Dilute solution properties of hyaluronic acid fractions.
    Biopolymers. 1970;9(7):799-810 PMID: 5424507
  33. Correlations between stiffness and the chemical constituents of cartilage on the human femoral head.
    Biochim Biophys Acta. 1970 Jul 21;215(1):70-7 PMID: 4250263
  34. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
    Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
  35. Properties of fractionated chondroitin sulphate from ox nasal septa.
    Biochem J. 1971 May;122(4):477-85 PMID: 5123881
  36. Hyaluronate in morphogenesis: inhibition of chondrogenesis in vitro.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1384-6 PMID: 4504347
  37. Stimulation of chondromucoprotein synthesis in chondrocytes by extracellular chondromucoprotein.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2069-72 PMID: 4506074
  38. The specific interaction of hyaluronic acid with cartillage proteoglycans.
    Biochim Biophys Acta. 1972 Sep 15;279(2):401-5 PMID: 4263642
  39. Viscoelastic properties of proteoglycan solutions with varying proportions present as aggregates.
    J Orthop Res. 1987;5(1):36-46 PMID: 3819910
  40. The inhibition of sulphate incorporation in isolated adult chondrocytes by hyaluronic acid.
    FEBS Lett. 1973 Nov 15;37(1):42-6 PMID: 4753758
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500435
Published
1988-03-01
Pages
435-41
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148875
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2500435 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2