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PMID: 3353381 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunological lesions in human uracil DNA glycosylase: association with Bloom syndrome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 7 ·1988-04-00 ·Pages 2339-43

Seal G, Brech K, Karp SJ, Cool BL, Sirover MA

Abstract

Three monoclonal antibodies that react with uracil DNA glycosylase of normal human placenta were tested to determine whether one of the antibodies could be used as a negative marker for Bloom syndrome. As defined by enzyme-linked immunosorbent assay, monoclonal antibody 40.10.09, which reacts with normal human glycosylase, neither recognized nor inhibited native uracil DNA glycosylase from any of five separate Bloom syndrome cell strains. Immunoblot analyses demonstrated that the denatured glycosylase protein from all five Bloom syndrome cell strains was immunoreactive with the 40.10.09 antibody. Further, each native enzyme was immunoreactive with two other anti-human placental uracil DNA glycosylase monoclonal antibodies. In contrast, ELISA reactivity was observed with all three monoclonal antibodies in reactions of glycosylases from 5 normal human cell types and 13 abnormal human cell strains. These results experimentally verify the specificity of the aberrant reactivity of the Bloom syndrome uracil DNA glycosylase. The possibility arises that determination of the lack of immunoreactivity with antibody 40.10.09 may have value in the early diagnosis of Bloom syndrome.

MeSH Terms
Antibodies, Monoclonal/immunology Bloom Syndrome/diagnosis,enzymology,immunology Cell Line DNA Glycosylases DNA Repair Diagnosis, Differential Enzyme-Linked Immunosorbent Assay Fibroblasts/enzymology Genetic Diseases, Inborn/diagnosis,enzymology Humans N-Glycosyl Hydrolases/genetics,immunology Uracil-DNA Glycosidase
Chemicals
Antibodies, Monoclonal DNA Glycosylases N-Glycosyl Hydrolases Uracil-DNA Glycosidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Seal G
Fels Research Institute, Temple University School of Medicine, Philadelphia, PA 19140.
Brech K
Karp S J
Cool B L
Sirover M A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.7.2339
Published
1988-04-00
Pages
2339-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279987
Subset
IM
Grants
NCI NIH HHS · CA-12227 · United States
NCI NIH HHS · CA-29414 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.7.2339 PMC Full Text View journal details
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