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PMID: 3349874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure and function of Y chromosomal DNA. I. Sequence organization and localization of four families of repetitive DNA on the Y chromosome of Drosophila hydei.

Chromosoma ·Vol. 96 ·No. 2 ·1988-00-00 ·Pages 145-58

Wlaschek M, Awgulewitsch A, Bünemann H

Abstract

The sequence organization of four different families of Y chromosomal repetitive DNA is characterized at three levels of spatial extension along the Y chromosome of Drosophila hydei. At the lowest level of resolution, DNA blot analysis of Y chromosomal fragments of different lengths and in situ hybridization experiments on metaphase chromosomes demonstrate the clustering of each particular sequence family within one defined region of the chromosome. At a higher level of resolution, family specific repeats can be detected within these clusters by crosshybridization within 10-20 kb long continuous stretches of cloned DNA in EMBL3 phages. At the highest level of resolution, detailed sequence analysis of representative subclones about 1 kb in length reveals a satellite-like head to tail arrangement of family specific degenerated subrepeats as the building scheme common to all four families. Our results provide the first comparative sequence analysis of three novel families of repetitive DNA on the long arm of the Y chromosome of D. hydei. Additional data are presented which support the existence of two related subfamilies of repetitive DNA on the short arm of the Y chromosome.

MeSH Terms
Animals Base Sequence DNA, Satellite/analysis Drosophila/genetics Male Molecular Sequence Data Repetitive Sequences, Nucleic Acid Y Chromosome/ultrastructure
Chemicals
DNA, Satellite
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wlaschek M
Institut für Genetik der Universität, Düsseldorf, Federal Republic of Germany.
Awgulewitsch A
Bünemann H
References (37)
37 references, click to expand
  1. Molecular arrangement and evolution of heterochromatic DNA.
    Annu Rev Genet. 1980;14:121-44 PMID: 6260016
  2. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  3. Evolution of Y chromosomal lampbrush loop DNA sequences of Drosophila.
    Chromosoma. 1986;94(5):367-76 PMID: 2881740
  4. Hoechst 33258 fluorescent staining of Drosophila chromosomes.
    Chromosoma. 1975;49(4):333-56 PMID: 48449
  5. Molecular cloning of microdissected lampbrush loop DNA sequences of Drosophila hydei.
    EMBO J. 1983;2(10):1741-6 PMID: 16453474
  6. Highly repetitive DNA sequences in the genome of Drosophila hydei. I. Preferential localization in the X chromosomal heterochromatin.
    J Mol Biol. 1972 Nov 14;71(2):407-17 PMID: 4635991
  7. Complementation of genetic activity in translocated fragmentsof the Y chromosome in Drosophila hydei.
    Genetics. 1967 Jun;56(2):283-95 PMID: 6035600
  8. Isolation of Y-chromosomal repetitive DNA sequences of Drosophila hydei via enrichment of chromosome-specific sequences by heterogeneous hybridization between female and male DNA.
    J Biochem Biophys Methods. 1986 Jan;12(1-2):37-50 PMID: 3944419
  9. DNA clones and RNA transcripts of four lampbrush loops from the Y chromosome of Drosophila hydei.
    Cell. 1983 Jan;32(1):191-9 PMID: 6186391
  10. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  11. Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.
    J Histochem Cytochem. 1978 Aug;26(8):677-9 PMID: 99471
  12. Visualization of gene transcription in spermatocytes of Drosophila hydei.
    Chromosoma. 1975 Dec 29;53(4):371-9 PMID: 1212903
  13. Genetic activities of the Y chromosome in Drosophila during spermatogenesis.
    Adv Genet. 1968;14:171-223 PMID: 4884781
  14. Y chromosome function and spermatogenesis in Drosophila hydei.
    Adv Genet. 1985;23:179-234 PMID: 3887859
  15. Structure and function of Y chromosomal DNA. II. Analysis of lampbrush loop associated transcripts in nuclei of primary spermatocytes of Drosophila hydei by in situ hybridization using asymmetric RNA probes of four different families of repetitive DNA.
    Chromosoma. 1988;96(2):159-70 PMID: 2450723
  16. Two separated nucleolus organizers on the Drosophila hydei Y chromosome.
    Mol Gen Genet. 1975;137(4):365-8 PMID: 1186660
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus.
    Cell. 1981 Jun;24(3):649-59 PMID: 6166385
  19. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  20. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  21. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  22. Lampbrush loop-specific protein of Drosophila hydei.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3404-8 PMID: 6587357
  23. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  24. Lampbrush chromosome loop-specificity of transcript morphology in spermatocyte nuclei of Drosophila hydei.
    EMBO J. 1984 Dec 1;3(12):2845-9 PMID: 16453579
  25. The molecular organization of the Antennapedia locus of Drosophila.
    Cell. 1983 Dec;35(3 Pt 2):763-76 PMID: 6418389
  26. Genetic function correlated with unfolding of lampbrush loops by the Y chromosome in spermatocytes of Drosophila hydei.
    Mol Gen Genet. 1970;106(4):328-46 PMID: 5481022
  27. Y chromosomal DNA of Drosophila hydei.
    J Mol Biol. 1983 Jun 15;167(1):37-56 PMID: 6306254
  28. Identification of cloned Y chromosomal DNA sequences from a lampbrush loop of Drosophila hydei.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5132-6 PMID: 6291024
  29. Molecular evidence for partial inactivation of Y loops in T(X:Y)56 males from D. hydei.
    Mol Gen Genet. 1985;199(1):46-52 PMID: 3858632
  30. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  31. Ecdysone-stimulated RNA synthesis in imaginal discs of Drosophila melanogaster. Assay by in situ hybridization.
    Chromosoma. 1976 Oct 12;58(1):87-99 PMID: 826375
  32. Cytological dissection of sex chromosome heterochromatin of Drosophila hydei.
    Chromosoma. 1981;84(3):391-403 PMID: 6173175
  33. [STRUCTURE-DIFFERENTIATION IN THE Y-CHROMOSOME OF DROSOPHILA HYDEI AND ITS RELATION TO GENE ACTIVITY. 3. SEQUENCE AND LOCALIZATION OF THE LOOP ORGANIZERS].
    Chromosoma. 1965 Feb 16;16:222-48 PMID: 14340397
  34. Origin and evolution of the transcribed repeated sequences of the Y chromosome lampbrush loops of Drosophila hydei.
    Proc Natl Acad Sci U S A. 1986 Jan;83(1):125-9 PMID: 16593641
  35. Differential chromosomal distribution of ribonucleoprotein antigens in nuclei of Drosophila spermatocytes.
    J Cell Biol. 1986 Dec;103(6 Pt 1):2113-9 PMID: 3536959
  36. A procedure for the cloning and identification of Y-specific middle repetitive sequences in Drosophila hydei.
    J Mol Biol. 1979 Sep 15;133(2):267-77 PMID: 537053
  37. Nucleic acid hybridization to the DNA of cytological preparations.
    Methods Cell Biol. 1975;10:1-16 PMID: 1102840
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00331047
Published
1988-00-00
Pages
145-58
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331047 View journal details
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