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PMID: 334723 Published · ppublish English Journal Article

Genetic locus (ompB) affecting a major outer-membrane protein in Escherichia coli K-12.

Journal of bacteriology ·Vol. 132 ·No. 1 ·1977-10-00 ·Pages 23-7

Sarma V, Reeves P

Abstract

Three multiply colicin-tolerant mutants in Escherichia coli K-12 from the TolIV, TolXIV, and TolXV phenotypic groups, all lacking or having only trace amounts of protein 1, a major outer-membrane protein, were mapped by Hfr crosses, and the position on the chromosome was confirmed by cotransduction with nearby markers. The mutations were located near malQP in the 74-min region of the E. coli chromosome. This locus is designated ompB, and analysis of data from two three-point crosses determined the linear sequence of genes to be aroB-ompB-malQP-glpD.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Cell Wall/metabolism Chromosome Mapping Colicins/pharmacology Drug Resistance, Microbial Escherichia coli/drug effects,genetics,metabolism Genes Mutation Transduction, Genetic
Chemicals
Bacterial Proteins Colicins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sarma V
Reeves P
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.132.1.23-27.1977
Published
1977-10-00
Pages
23-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221821
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.132.1.23-27.1977 PMC Full Text View journal details
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