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PMID: 3342063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Giant polypeptides of skeletal muscle titin: sedimentation equilibrium in guanidine hydrochloride.

Biochemical and biophysical research communications ·Vol. 150 ·No. 3 ·1988-02-15 ·Pages 1155-61

Kurzban GP, Wang K

Abstract

Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1/S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 X 10(6).

MeSH Terms

Animals Chromatography, Gel Connectin Electrophoresis, Polyacrylamide Gel Guanidine Guanidines Molecular Weight Muscle Proteins/isolation & purification Muscles/analysis Protein Denaturation Protein Kinases Rabbits Ultracentrifugation

Chemicals

Connectin Guanidines Muscle Proteins Protein Kinases Guanidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kurzban G P

Clayton Foundation Biochemical Institute, Department of Chemistry, Austin, Texas.

Wang K

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(88)90750-4

Published

1988-02-15

Pages

1155-61

Language

English

Region

United States

NLM ID

0372516

Subset

Grants

NCI NIH HHS · CA 09182 · United States

NIDDK NIH HHS · DK 20270 · United States

External Links

PubMed source DOI: 10.1016/0006-291x(88)90750-4 View journal details

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Giant polypeptides of skeletal muscle titin: sedimentation equilibrium in guanidine hydrochloride.

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