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PMID: 3340554 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes.

Nucleic acids research ·Vol. 16 ·No. 2 ·1988-01-25 ·Pages 751-62

Tajmir-Riahi HA, Langlais M, Savoie R

Abstract

The interaction of calf-thymus DNA with Cu(II) and Pb(II) ions has been investigated in H2O and D2O solutions at physiological pH, using laser Raman spectroscopy. The results confirm the destabilizing effect of Cu2+ ions, which are shown to bind strongly to the guanine and cytidine bases, perturbing the A-T base pairs and disrupting the double-helical structure of DNA, whose conformation is markedly altered by these interactions. Earlier claims that Pb2+ ions destabilize DNA are not supported by the present study. These ions are found to interact only weakly with the nucleic bases, binding to the N7 position of the guanine bases and also interacting with the A-T pairs. Both types of ions are found to interact with the charged phosphate groups of DNA, although these sites are preferred over the nucleic bases by Pb2+ ions.

MeSH Terms

Copper DNA Lead Metals Nucleic Acid Conformation Solutions Spectrum Analysis, Raman

Chemicals

Metals Solutions Lead Copper DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tajmir-Riahi H A

Department of Chemistry, Laval University, Quebec, Canada.

Langlais M

Savoie R

References (17)

17 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.2.751

Published

1988-01-25

Pages

751-62

Language

English

Region

England

NLM ID

0411011

PMCID

PMC334689

Subset

External Links

PubMed source DOI: 10.1093/nar/16.2.751 PMC Full Text View journal details

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A laser Raman spectroscopic study of the interaction of calf-thymus DNA with Cu(II) and Pb(II) ions: metal ion binding and DNA conformational changes.

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