The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.

Histochemistry ·Vol. 53 ·No. 1 ·1977-07-18 ·Pages 57-62

Bosman FT, Lindeman J, Kuiper G, van der Wal A, Kreunig J

Abstract

The influence of different fixation methods on the results of immunoperoxydase staining of immunoglobulin and gastrin producing cells in gastric and duodenal mucosa was investigated. An indirect method was used on paraffin sections. It appeared that that fixatives containing sublimate gave the most consistent results, a sublimate-formaldehyde mixture being the best.

MeSH Terms

Fixatives/pharmacology Formaldehyde/pharmacology Gastric Mucosa/cytology Gastrins Humans Immunoenzyme Techniques Intestinal Mucosa/cytology Plasma Cells/analysis,drug effects

Chemicals

Fixatives Gastrins Formaldehyde

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bosman F T

Lindeman J

Kuiper G

van der Wal A

Kreunig J

References (13)

13 references, click to expand

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Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00511210

Published

1977-07-18

Pages

57-62

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00511210

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The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.

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