Home LiteratureArticle Details
PMID: 3321052 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Comparative mutational analysis of wild-type and stretched tRNA3(Leu) gene promoters.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8763-7

Fabrizio P, Coppo A, Fruscoloni P, Benedetti P, Di Segni G, Tocchini-Valentini GP

Abstract

We demonstrate that, when the yeast tRNA(3Leu) gene is stretched so that the distance between the two portions of the intragenic promoter is increased to 365 base pairs, the A and B blocks remain functional. Mutations in the A block, which show a weak phenotype when inserted in the wild type, exert a dramatic effect when inserted into the stretched gene. Experiments with extensively purified transcription factor tau indicate that the tau B-B block interaction is not influenced by A-B distance; only the ability of tau A to interact with A block sequences is affected, possibly because of the additional free-energy cost of forming a large loop of the intervening DNA.

MeSH Terms
Base Sequence Binding Sites DNA Mutational Analysis DNA, Fungal/genetics DNA-Binding Proteins/physiology Gene Expression Regulation Molecular Sequence Data Promoter Regions, Genetic RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Leu/genetics Saccharomyces cerevisiae/genetics Transcription Factors/physiology Transcription, Genetic
Chemicals
DNA, Fungal DNA-Binding Proteins RNA, Transfer, Amino Acid-Specific RNA, Transfer, Leu Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fabrizio P
Dipartimento di Biologia Cellulare e dello Sviluppo, Università degli Studi La Sapienza, Rome, Italy.
Coppo A
Fruscoloni P
Benedetti P
Di Segni G
Tocchini-Valentini G P
References (25)
25 references, click to expand
  1. Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C.
    J Biol Chem. 1986 Apr 25;261(12):5275-82 PMID: 3633923
  2. A split binding site for transcription factor tau on the tRNA3Glu gene.
    EMBO J. 1985 Feb;4(2):491-500 PMID: 2862029
  3. Selective proteolysis defines two DNA binding domains in yeast transcription factor tau.
    Nature. 1986 Sep 11-17;323(6084):176-8 PMID: 3528868
  4. Gene size differentially affects the binding of yeast transcription factor tau to two intragenic regions.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8768-72 PMID: 2827154
  5. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
    Nucleic Acids Res. 1978 Sep;5(9):3157-70 PMID: 212715
  6. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
    Cell. 1978 Nov;15(3):1077-86 PMID: 569551
  7. Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2564-8 PMID: 6994101
  8. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  9. Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity.
    Cell. 1980 Jul;20(3):701-9 PMID: 6998562
  10. Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.
    Nucleic Acids Res. 1981 Aug 11;9(15):3647-56 PMID: 7279669
  11. Deletion of the 3' half of the yeast tRNA-Leu3 gene does not abolish promotor function in vitro.
    Cell. 1981 Dec;27(2 Pt 1):371-9 PMID: 6277504
  12. Specific transcription of homologous class III genes in yeast-soluble cell-free extracts.
    J Biol Chem. 1982 Jul 25;257(14):8432-41 PMID: 7045122
  13. Specific interactions of Saccharomyces cerevisiae proteins with a promoter region of eukaryotic tRNA genes.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6191-5 PMID: 6755466
  14. Mutation in the a block of the yeast tRNAleu3 gene that allows transcription but abolishes splicing and 5'-end maturation.
    Cell. 1983 Jan;32(1):67-76 PMID: 6337723
  15. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription.
    Nucleic Acids Res. 1982 Dec 20;10(24):8127-43 PMID: 6298710
  16. Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
    Cell. 1983 Mar;32(3):725-33 PMID: 6299574
  17. Role of RNA structure in splicing: excision of the intervening sequence in yeast tRNA3leu is dependent on the formation of a D stem.
    Cell. 1983 Nov;35(1):109-15 PMID: 6194896
  18. tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter.
    Cell. 1983 Nov;35(1):117-25 PMID: 6226365
  19. Isolation of a class C transcription factor which forms a stable complex with tRNA genes.
    EMBO J. 1984 Feb;3(2):343-50 PMID: 6370678
  20. Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.
    EMBO J. 1984 Apr;3(4):847-53 PMID: 6233142
  21. Compilation of sequences of tRNA genes.
    Nucleic Acids Res. 1984;12 Suppl:r59-131 PMID: 6728687
  22. Effects of temperature and single-stranded DNA on the interaction of an RNA polymerase III transcription factor with a tRNA gene.
    Cell. 1985 Feb;40(2):311-7 PMID: 3967295
  23. Structural features of yeast tRNA genes which affect transcription factor binding.
    EMBO J. 1984 Dec 1;3(12):2793-800 PMID: 6396080
  24. A large region controls tRNA gene transcription.
    J Mol Biol. 1985 May 25;183(2):153-63 PMID: 3847510
  25. Gene regulation by proteins acting nearby and at a distance.
    Nature. 1986 Aug 21-27;322(6081):697-701 PMID: 3018583
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8763
Published
1987-12-00
Pages
8763-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299630
Subset
IM
Databases
GENBANK
M19060
External Links
PubMed source DOI: 10.1073/pnas.84.24.8763 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2