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PMID: 3319802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Review

Detection of lipid hydroperoxides and hydrogen peroxide at picomole levels by an HPLC and isoluminol chemiluminescence assay.

Free radical biology & medicine ·Vol. 3 ·No. 5 ·1987-00-00 ·Pages 359-61

Yamamoto Y, Ames BN

Abstract

An isoluminol assay is utilized for the detection of hydrogen peroxide and lipid hydroperoxides in biological samples. The combination of this assay as a post-column detection for HPLC avoids interference of antioxidants and enables characterization of hydroperoxides at picomole levels. Two useful HPLC conditions for the separation of hydrogen peroxide, lipid hydroperoxides, antioxidants, and unoxidized lipids are described.

MeSH Terms

Animals Chromatography, High Pressure Liquid Humans Hydrogen Peroxide/analysis,blood Lipid Peroxides/analysis,blood,isolation & purification Luminescent Measurements

Chemicals

Lipid Peroxides Hydrogen Peroxide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Yamamoto Y

Department of Biochemistry, University of California, Berkeley 94720.

Ames B N

Article Info

Journal

Free radical biology & medicine

Abbr.

Free Radic Biol Med

ISSN

0891-5849

DOI

10.1016/s0891-5849(87)80048-5

Published

1987-00-00

Pages

359-61

Language

English

Region

United States

NLM ID

8709159

Subset

Grants

NCI NIH HHS · CA39910 · United States

NIEHS NIH HHS · ES01896 · United States

NIGMS NIH HHS · GM19993 · United States

External Links

PubMed source DOI: 10.1016/s0891-5849(87)80048-5 View journal details

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Detection of lipid hydroperoxides and hydrogen peroxide at picomole levels by an HPLC and isoluminol chemiluminescence assay.

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