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PMID: 331081 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The regulation of RNA synthesis in yeast. I: Starvation experiments.

Molecular & general genetics : MGG ·Vol. 154 ·No. 2 ·1977-07-20 ·Pages 145-53

Oliver SG, McLaughlin CS

Abstract

The synthesis of tRNA in yeast is shown to be under separate control to that of rRNA during amino acid and nitrogen starvation. Inhibitors of the elongation and termination steps of protein synthesis were found to stimulate the synthesis of tRNA in starved yeast cells. This effect appeared to be due to the "trickle-charging" of tRNA. Two inhibitors of early steps in the initiation of protein synthesis were found to be unable to stimulate RNA synthesis in starved cells. It is proposed that yeast tRNA synthesis is under autoregulatory control and that the level of tRNA charging and the mRNA-ribosome complex are important components of this control system.

MeSH Terms
Amino Acids/metabolism Cycloheximide/pharmacology Mutation Nitrogen/metabolism Protein Biosynthesis RNA, Ribosomal/biosynthesis RNA, Transfer/biosynthesis Saccharomyces cerevisiae/drug effects,metabolism Temperature Transcription, Genetic
Chemicals
Amino Acids RNA, Ribosomal RNA, Transfer Cycloheximide Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oliver S G
McLaughlin C S
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330830
Published
1977-07-20
Pages
145-53
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330830
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