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PMID: 33084111 Published · ppublish English Journal Article

ALG3 contributes to the malignant properties of OSCC cells by regulating CDK-Cyclin pathway.

Oral diseases ·Vol. 27 ·No. 6 ·2021-09-00 ·Pages 1426-1434

Shao P, Wei C, Wang Y

Abstract

In this study, we planned to investigate the function and potential mechanisms of Alpha-1,3-mannosyltransferase (ALG3) in oral squamous cell carcinoma (OSCC). Data from TCGA were used to analyze ALG3 expression and its effect on the prognosis of patients with OSCC. KEGG enrichment analysis was applied to explore the pathways related to ALG3. ALG3 expression was measured by qPCR and Western blot. Cell counting kit-8, colony formation, and transwell assays were implemented to detect the effects of ALG3 on malignant biological properties of OSCC cells. The expression of key proteins related to CDK-Cyclin pathway was detected by Western blot. The expression of ALG3 in OSCC samples was higher than that of the control samples, and the increase of ALG3 expression was related to unfavorable prognosis of OSCC patients. Additionally, the elevated expression of ALG3 was associated with pathological stage, lymph node metastasis, and primary lesion in OSCC patients. ALG3 depletion blocked the growth and movement of OSCC cells, while over-expression ALG3 reversed these phenomena. Moreover, exhaustion of ALG3 resulted in decreased expression of MCM7/CCNB2/CDK1/PCNA, while these phenomena were inversed after ALG3 up-regulation. The enhancement of ALG3 expression promoted the aggressive biological behaviors of OSCC cells probably by promoting CDK-Cyclin pathway.

Keywords
ALG3 cell cycle invasion oral squamous cell carcinoma prognosis
MeSH Terms
Carcinoma, Squamous Cell/genetics Cell Line, Tumor Cell Movement Cell Proliferation Cyclins Head and Neck Neoplasms Humans Mannosyltransferases Mouth Neoplasms/genetics Proliferating Cell Nuclear Antigen Squamous Cell Carcinoma of Head and Neck
Chemicals
Cyclins Proliferating Cell Nuclear Antigen ALG3 protein, human Mannosyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shao Peihong
Stomatology Wards of Tengzhou Central People's Hospital in Shandong Province, Tengzhou, China.
Wei Chengshi
Stomatology Department, Liaocheng People's Hospital, Liaocheng, China.
Wang Yun ORCID
Stomatology Department, Liaocheng People's Hospital, Liaocheng, China.
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Article Info
Journal
Oral diseases
Abbr.
Oral Dis
ISSN
1601-0825
DOI
10.1111/odi.13687
Published
2021-09-00
Epub
2020-00-08
Pages
1426-1434
Language
English
Region
Denmark
NLM ID
9508565
External Links
PubMed source DOI: 10.1111/odi.13687 View journal details
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