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PMID: 3302673 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Homologous recombination between single-stranded DNA and chromosomal genes in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 7 ·No. 7 ·1987-07-00 ·Pages 2329-34

Simon JR, Moore PD

Abstract

Transformation of Saccharomyces cerevisiae strains was examined by using the URA3 and TRP1 genes cloned into M13 vectors in the absence of sequences capable of promoting autonomous replication. These constructs transform S. cerevisiae cells to prototrophy by homologous recombination with the resident mutant gene. Single-stranded DNA was found to transform S. cerevisiae cells at efficiencies greater than that of double-stranded DNA. No conversion of single-stranded transforming DNA into duplex forms could be detected during the transformation process, and we conclude that single-stranded DNA may participate directly in recombination with chromosomal sequences. Transformation with single-stranded DNA gave rise to both gene conversion and reciprocal exchange events. Cotransformation with competing heterologous single-stranded DNA specifically inhibited transformation by single-stranded DNA, suggesting that one of the components in the transformation-recombination process has a preferential affinity for single-stranded DNA.

MeSH Terms
Binding, Competitive Chromosomes Cloning, Molecular DNA, Fungal/genetics DNA, Single-Stranded/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics Transformation, Genetic
Chemicals
DNA, Fungal DNA, Single-Stranded
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simon J R
Moore P D
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21 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.7.2329-2334.1987
Published
1987-07-00
Pages
2329-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365363
Subset
IM
Grants
NCRR NIH HHS · SO7 RR 05369 · United States
NCI NIH HHS · T32 CA 09318 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.7.2329-2334.1987 PMC Full Text View journal details
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