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PMID: 3301806 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activation of the xylDLEGF promoter of the TOL toluene-xylene degradation pathway by overproduction of the xylS regulatory gene product.

Journal of bacteriology ·Vol. 169 ·No. 8 ·1987-08-00 ·Pages 3581-6

Spooner RA, Bagdasarian M, Franklin FC

Abstract

The xylS regulatory gene of the Pseudomonas putida TOL plasmid (pWWO) has been cloned under the transcriptional control of the Escherichia coli tac promoter in a broad-host-range controlled-expression vector. Induction with isopropylthiogalactoside allowed overproduction and characterization of the xylS product by specific interaction with the TOL meta-cleavage pathway operator-promoter region (OP2) in vivo in E. coli. Examination of plasmid-specified polypeptides in E. coli maxicells led to identification of the xylS product as a 36-kilodalton polypeptide. The operator sequences required for xylS interactions lay upstream of the OP2 transcriptional start, and the xylS gene product recognized this region even in the absence of known coinducers.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Cloning, Molecular Escherichia coli/genetics,metabolism Genes, Bacterial Genes, Regulator Plasmids Promoter Regions, Genetic Pseudomonas/genetics,metabolism Toluene/metabolism Xylenes/metabolism
Chemicals
Bacterial Proteins Xylenes Toluene
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Spooner R A
Bagdasarian M
Franklin F C
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.8.3581-3586.1987
Published
1987-08-00
Pages
3581-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212435
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.8.3581-3586.1987 PMC Full Text View journal details
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