Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens.

The EMBO journal ·Vol. 6 ·No. 6 ·1987-06-00 ·Pages 1673-6

Krimpenfort P, Rudenko G, Hochstenbach F, Guessow D, Berns A, Ploegh H

Abstract

In man a number of diseases are associated with certain alleles of MHC antigens. The most pronounced example is ankylosing spondylitis, which is strongly associated with HLA-B27. As a first step towards a model system to study the basis of this association, transgenic mice were generated that showed cell surface expression of the HLA-B27 antigen biochemically indistinguishable from HLA-B27 antigen expressed on human cells. This result was obtained by crossing two independently derived strains of mice, one of which is transgenic for the HLA-B27 heavy chain gene, and the other carrying and expressing the human beta 2m gene. Examination of HLA-B27 and human beta 2m mRNA in various tissues shows the two genes to be expressed in a coordinate fashion. The mRNA levels follow those of endogenous H-2 Class I genes.

MeSH Terms

Animals Cloning, Molecular Crosses, Genetic Female Genes HLA Antigens/genetics HLA-B27 Antigen Histocompatibility Antigens Humans Lymphocytes/immunology Macromolecular Substances Male Mice beta 2-Microglobulin/genetics

Chemicals

HLA Antigens HLA-B27 Antigen Histocompatibility Antigens Macromolecular Substances beta 2-Microglobulin

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Krimpenfort P

Rudenko G

Hochstenbach F

Guessow D

Berns A

Ploegh H

References (17)

17 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-06-00

Pages

1673-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553540

Subset

External Links

PubMed source PMC Full Text View journal details

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Crosses of two independently derived transgenic mice demonstrate functional complementation of the genes encoding heavy (HLA-B27) and light (beta 2-microglobulin) chains of HLA class I antigens.

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