Home LiteratureArticle Details
PMID: 330055 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histamine-receptor leucocytes (HRL). Organ and lymphoid subpopulation distribution in man.

Clinical and experimental immunology ·Vol. 28 ·No. 3 ·1977-06-00 ·Pages 394-9

Saxon A, Morledge VD, Bonavida B

Abstract

The frequency of lymphoid cells with a membrane receptor for histamine was determined in various lymphoid organs in man using a histamine-rosette assay. Thymus had very low numbers of histamine-receptor cells while lymph node and peripheral blood had increasing percentages. Through a combination of cell separation techniques, we demonstrated that about one third (1/3) of peripheral blood B lymphocytes and macrophages carry histamine receptors. Immature B cells or null cells (E-rosette and membrane-immunoglobulin-negative) do not have this receptor. Only 10% of peripheral blood T lymphocytes formed histamine rosettes. That these histamine receptor T lymphocytes are a subpopulation representing the differentiated suppressor/cytotoxic T cells is suggested by evidence showing complete removal of histamine receptor T lymphocytes on nylon wool adherence columns. Thus, the histamine receptor is expressed on differentiated B and T lymphocytes and may serve as a marker for developed suppressor/cytotoxic T cells in man.

MeSH Terms
B-Lymphocytes/immunology Cell Separation Clone Cells Humans Immunologic Techniques Leukocyte Count Lymph Nodes/immunology Receptors, Histamine T-Lymphocytes/immunology Thymus Gland/immunology
Chemicals
Receptors, Histamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saxon A
Morledge V D
Bonavida B
References (21)
21 references, click to expand
  1. Single step separation of human T and B cells using AET treated srbc rosettes.
    J Immunol Methods. 1976;12(3-4):285-8 PMID: 1085784
  2. Cytochemical identification of monocytes and granulocytes.
    Am J Clin Pathol. 1971 Mar;55(3):283-90 PMID: 5549896
  3. Suppressor cell induction in vitro. II. Cellular requirements of suppressor cell induction.
    Eur J Immunol. 1976 Apr;6(4):302-5 PMID: 1086779
  4. Severe combined immunodeficiency with B lymphocytes: in vitro correction of defective immunoglobulin production by addition of normal T lymphocytes.
    Clin Exp Immunol. 1976 Oct;26(1):1-10 PMID: 1087209
  5. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.
    Scand J Clin Lab Invest Suppl. 1968;97:77-89 PMID: 4179068
  6. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.
    J Exp Med. 1972 Aug 1;136(2):207-15 PMID: 4114496
  7. Modulation of inflammation and immunity by cyclic AMP.
    Science. 1974 Apr 5;184(4132):19-28 PMID: 4131281
  8. Receptors for histamine can be detected on the surface of selected leukocytes.
    Science. 1972 Aug 25;177(4050):707-9 PMID: 4403369
  9. Increase in histamine receptors on thymus-derived effector lymphocytes during the primary immune response to alloantigens.
    Nature. 1973 Aug 3;244(5414):284-7 PMID: 4147637
  10. Histamine receptor-bearing leukocytes (HRL). I. Detection of histamine receptor-bearing cells by rosette formation with histamine-coated erythrocytes.
    J Immunol. 1974 Nov;113(5):1544-52 PMID: 4213629
  11. Purification of human T and B lymphocytes.
    J Immunol. 1974 Jan;112(1):420-3 PMID: 4544194
  12. Antibody dependent cellular cytotoxicity: cytotoxicity mediated by non t-lymphocytes.
    Clin Immunol Immunopathol. 1974 Nov;3(2):193-200 PMID: 4548867
  13. Regulation of antibody response by cells expressing histamine receptors.
    J Exp Med. 1972 Nov 1;136(5):1302-7 PMID: 4563150
  14. The percentage of monocytes among "mononuclear" cell fractions obtained from normal human blood.
    J Immunol. 1974 Jan;112(1):234-40 PMID: 4521396
  15. The separation and reactivity in vitro of a subpopulation of human lymphocytes which bind histamine. Correlation of histamine reactivity with cellular maturation.
    Cell Immunol. 1976 Jun 15;24(2):250-69 PMID: 132992
  16. IgG on lymphocyte surfaces; technical problems and the significance of a third cell population.
    J Immunol. 1975 Apr;114(4):1210-2 PMID: 804005
  17. Suppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients.
    J Clin Invest. 1976 Jul;58(1):109-22 PMID: 1084353
  18. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen.
    J Exp Med. 1975 Jun 1;141(6):1376-89 PMID: 1092798
  19. Immunologic functions of isolated human lymphocyte subpopulations. VI. Further characterization of the surface Ig negative, E rosette negative (null cell) subset.
    J Immunol. 1975 Dec;115(6):1483-7 PMID: 1102600
  20. Distribution of cells binding erythrocyte-antibody (EA) complexes in human lymphoid populations.
    Scand J Immunol. 1976;5(3):221-31 PMID: 1084016
  21. A simple rosette assay for demonstration of complement receptor sites using complement-coated zymosan beads.
    Eur J Immunol. 1976 Jun;5(6):432-5 PMID: 1086242
Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1977-06-00
Pages
394-9
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1541015
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2