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PMID: 3290644 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of expression and activity of the yeast transcription factor ADR1.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 1868-76

Blumberg H, Hartshorne TA, Young ET

Abstract

Disruption of ADR1, a positive regulatory gene in the yeast Saccharomyces cerevisiae, abolished derepression of ADH2 but did not affect glucose repression of ADH2 or cell viability. The ADR1 mRNA was 5 kilobases long and had an unusually long leader containing 509 nucleotides. ADR1 mRNA levels were regulated by the carbon source in a strain-dependent fashion. beta-Galactosidase levels measured in strains carrying an ADR1-lacZ gene fusion paralleled ADR1 and ADR1-lacZ mRNA levels, indicating a lack of translational regulation of ADR1 mRNA. ADH2 was regulated by the carbon source to the same extent in all strains examined and showed complete dependence on ADR1 as well. The expression of ADR1 mRNA and an ADR1-beta-galactosidase fusion protein during glucose repression suggested that the activity of the ADR1 protein is regulated at the posttranslational level to properly regulate ADH2 expression. The ADR1-beta-galactosidase fusion protein was able to activate ADH2 expression during glucose repression but showed significantly higher levels of activation upon derepression. A similar result was obtained when ADR1 was present on a multicopy plasmid. These results suggest that low-level expression of ADR1 is required to maintain glucose repression of ADH2 and are consistent with the hypothesis that ADR1 is regulated at the posttranslational level.

MeSH Terms
DNA-Binding Proteins Fungal Proteins/biosynthesis,genetics Gene Expression Regulation/drug effects Glucose/pharmacology Protein Processing, Post-Translational RNA, Fungal/metabolism RNA, Messenger/metabolism Recombinant Fusion Proteins/biosynthesis,genetics Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/biosynthesis,genetics
Chemicals
ADR1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins RNA, Fungal RNA, Messenger Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blumberg H
Department of Biochemistry, University of Washington, Seattle 98195.
Hartshorne T A
Young E T
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.1868-1876.1988
Published
1988-05-00
Pages
1868-76
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363364
Subset
IM
Grants
NIGMS NIH HHS · 5-T32-GM-07270 · United States
NIGMS NIH HHS · GM26079 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.5.1868-1876.1988 PMC Full Text View journal details
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