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PMID: 3287379 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Glucose stimulates proinsulin biosynthesis by a dose-dependent recruitment of pancreatic beta cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 11 ·1988-06-00 ·Pages 3865-9

Schuit FC, In't Veld PA, Pipeleers DG

Abstract

Glucose is a well-known stimulus of proinsulin biosynthesis. In purified beta cells, the sugar induces a 25-fold increase in the synthesis of insulin immunoreactive material over 60-min incubation. Autoradiographic analysis of the individual cells shows that this effect is achieved via dose-dependent recruitment of pancreatic beta cells to biosynthetic activity. Recruitment of beta cells is also seen in isolated islets exposed to glucose. The sigmoidal dose-response curve for glucose-induced proinsulin biosynthesis thus reflects a heterogeneous responsiveness of pancreatic beta cells rather than a progressively increasing activity of functionally homogeneous cells. Dose-dependent recruitment of functionally diverse cells may be a ubiquitous mechanism in tissue function.

MeSH Terms
Animals Autoradiography Cell Separation Cells, Cultured Dose-Response Relationship, Drug Glucose/pharmacology In Vitro Techniques Islets of Langerhans/cytology,metabolism Proinsulin/biosynthesis Rats
Chemicals
Proinsulin Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schuit F C
Department of Metabolism, Vrije Universiteit Brussel, Belgium.
In't Veld P A
Pipeleers D G
References (37)
37 references, click to expand
  1. Insulin biosynthesis: evidence for a precursor.
    Science. 1967 Aug 11;157(3789):697-700 PMID: 4291105
  2. The stimulus-secretion coupling of glucose-induced insulin release. XIV. Glucose regulation of insular biosynthetic activity.
    Endocrinology. 1973 Nov;93(5):1001-11 PMID: 4591649
  3. Functional subdivision of islets of Langerhans and possible role of D cells.
    Lancet. 1975 Dec 20;2(7947):1243-4 PMID: 53729
  4. Mitabolic adaptation to pregnancy shown by increased biosynthesis of insulin in islets of Langerhans isolated from pregnant rat.
    Nature. 1976 Aug 5;262(5568):501-2 PMID: 785279
  5. Fast motor units are not preferentially activated in rapid voluntary contractions in man.
    Nature. 1977 Jun 23;267(5613):717-9 PMID: 876393
  6. Secretory capability of islets transplanted intraportally in the diabetic rat.
    Diabetes. 1978 Aug;27(8):817-24 PMID: 98376
  7. Insulin release: the fuel hypothesis.
    Metabolism. 1979 Apr;28(4):373-86 PMID: 36543
  8. Translational control of proinsulin synthesis by glucose.
    Nature. 1980 Jan 3;283(5742):100-2 PMID: 6985712
  9. Intracellular degradation of insulin stores by rat pancreatic islets in vitro. An alternative pathway for homeostasis of pancreatic insulin content.
    J Biol Chem. 1980 Jul 10;255(13):6003-6 PMID: 6993463
  10. The effects of fasting and feeding on preproinsulin messenger RNA in rats.
    J Clin Invest. 1981 Apr;67(4):952-60 PMID: 6162861
  11. A method for the purification of single A, B and D cells and for the isolation of coupled cells from isolated rat islets.
    Diabetologia. 1981 Jun;20(6):654-63 PMID: 6114890
  12. Direct effect of glucose on the preproinsulin mRNA level in isolated pancreatic islets.
    Biochem Biophys Res Commun. 1982 Jun 30;106(4):1383-9 PMID: 7052089
  13. Islet cell analysis and purification by light scatter and autofluorescence.
    Biochem Biophys Res Commun. 1982 Jul 30;107(2):525-32 PMID: 6751329
  14. Autofluorescence-activated cell sorting of pancreatic islet cells: purification of insulin-containing B-cells according to glucose-induced changes in cellular redox state.
    Biochem Biophys Res Commun. 1983 Jul 29;114(2):835-42 PMID: 6349638
  15. Islet cell interactions with pancreatic B-cells.
    Experientia. 1984 Oct 15;40(10):1114-26 PMID: 6149149
  16. A new in vitro model for the study of pancreatic A and B cells.
    Endocrinology. 1985 Sep;117(3):806-16 PMID: 2862019
  17. Interplay of nutrients and hormones in the regulation of insulin release.
    Endocrinology. 1985 Sep;117(3):824-33 PMID: 2862021
  18. Pancreatic hormone receptors on islet cells.
    Endocrinology. 1985 Sep;117(3):841-8 PMID: 2990873
  19. Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. I. Effects of glucose and cyclic AMP on the transcription of insulin mRNA.
    J Biol Chem. 1985 Nov 5;260(25):13585-9 PMID: 2997172
  20. Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. II. Regulation of insulin mRNA stability.
    J Biol Chem. 1985 Nov 5;260(25):13590-4 PMID: 3902821
  21. Estrogen induction of very low density apolipoprotein II synthesis, a major avian liver yolk protein, involves the recruitment of hepatocytes.
    Endocrinology. 1986 Feb;118(2):538-44 PMID: 3510852
  22. Heterogeneity and contact-dependent regulation of hormone secretion by individual B cells.
    Exp Cell Res. 1986 Feb;162(2):507-20 PMID: 3510882
  23. Translational control of insulin biosynthesis. Evidence for regulation of elongation, initiation and signal-recognition-particle-mediated translational arrest by glucose.
    Biochem J. 1986 Apr 15;235(2):459-67 PMID: 3017307
  24. Reversible interconversion of two forms of a valyl-tRNA synthetase-containing protein complex.
    Science. 1986 Nov 28;234(4780):1111-4 PMID: 3535073
  25. Progressive recruitment of follicular cells with graded secretory responsiveness during stimulation of the thyroid gland by thyrotropin.
    Endocrinology. 1987 Jan;120(1):91-6 PMID: 3780572
  26. The biosociology of pancreatic B cells.
    Diabetologia. 1987 May;30(5):277-91 PMID: 3301479
  27. Electron microscopy of the normal islets of Langerhans; studies in the dog, rabbit, guinea pig and rat.
    Diabetes. 1957 Nov-Dec;6(6):498-507 PMID: 13473645
  28. Relation between size of neurons and their susceptibility to discharge.
    Science. 1957 Dec 27;126(3287):1345-7 PMID: 13495469
  29. FUNCTIONAL SIGNIFICANCE OF CELL SIZE IN SPINAL MOTONEURONS.
    J Neurophysiol. 1965 May;28:560-80 PMID: 14328454
  30. Effects of glucose concentration on incorporation of [3H]leucine into insulin using isolated mammalian islets of Langerhans.
    Biochim Biophys Acta. 1966 Dec 28;130(2):519-21 PMID: 5339718
  31. Glucose-induced electrical activity in pancreatic islet cells.
    J Physiol. 1970 Sep;210(2):255-64 PMID: 5501259
  32. Uptake of alanine, arginine and leucine by mammalian pancreatic beta-cells.
    Endocrinology. 1971 Dec;89(6):1432-9 PMID: 5001012
  33. Insulin biosynthesis. I. On the mechanism of glucose stimulation.
    J Biol Chem. 1972 Feb 25;247(4):1194-9 PMID: 4551514
  34. Effect of various concentrations of glucose on insulin biosynthesis.
    Endocrinology. 1972 Jul;91(1):309-11 PMID: 4554506
  35. A threshold distribution hypothesis for packet storage of insulin and its mathematical modeling.
    J Clin Invest. 1972 Aug;51(8):2047-59 PMID: 4559946
  36. The orderly recruitment of human motor units during voluntary isometric contractions.
    J Physiol. 1973 Apr;230(2):359-70 PMID: 4350770
  37. Anti-insulin serum coupled to Sepharose 4B as a tool for the investigation of insulin biosynthesis in the B-cells of obese hyperglycemic mice.
    Endocrinology. 1975 Nov;97(5):1241-7 PMID: 1102296
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.11.3865
Published
1988-06-00
Pages
3865-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280320
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.11.3865 PMC Full Text View journal details
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