Induction of alkaline phosphatase in Escherichia coli: effect of procaine hydrochloride.

Journal of bacteriology ·Vol. 131 ·No. 2 ·1977-08-00 ·Pages 431-7

Tribhuwan RC, Pradhan DS

Abstract

The effect of procaine hydrochloride, an anesthetic known to alter membrane structure, on the induced formation of alkaline phosphatase, a periplasmic enzyme, in Escherichia coli was investigated. Procaine hydrochloride specifically arrested the appearance of active alkaline phosphatase while permitting the induction of another enzyme, beta-galactosidase, which is internally localized. Evidence has been obtained to show that procaine hydrochloride does not arrest synthesis of inactive monomer subunits of the enzyme, indicating that the drug interferes in the conversion of monomer subunits to an active dimer enzyme.

MeSH Terms

Alkaline Phosphatase/biosynthesis Bacterial Proteins/biosynthesis Dose-Response Relationship, Drug Enzyme Induction/drug effects Escherichia coli/drug effects,enzymology Galactosidases/biosynthesis Procaine/pharmacology

Chemicals

Bacterial Proteins Procaine Alkaline Phosphatase Galactosidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tribhuwan R C

Pradhan D S

References (15)

15 references, click to expand

Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
Reactivation and hybridization of reduced alkaline phosphatase.
Proc Natl Acad Sci U S A. 1962 Jul 15;48:1230-7 PMID: 14464689
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
Isolation and properties of bacteria capable of high rates of beta-galactosidase synthesis.
Biochim Biophys Acta. 1962 Jan 22;55:152-63 PMID: 14448925
Mechanism of action of phenethyl alcohol: breakdown of the cellular permeability barrier.
J Bacteriol. 1967 Feb;93(2):560-6 PMID: 5335963
In vitro synthesis of Escherichia coli alkaline phosphatase monomers.
J Mol Biol. 1971 Jun 14;58(2):469-79 PMID: 4933414
Studies on the mechanism of action of local anesthetics with phospholipid model membranes.
Biochim Biophys Acta. 1972 Apr 18;265(2):169-86 PMID: 4555469
Effect of procaine hydrochloride on DNA repair in Escherichia coli.
Chem Biol Interact. 1975 Sep;11(3):173-8 PMID: 1098800
Alkaline phosphatase subunits and their dimerization in vivo.
J Bacteriol. 1968 Oct;96(4):1200-7 PMID: 4879556
Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli.
Biochim Biophys Acta. 1960 Mar 11;38:460-9 PMID: 13838951
Rat intestinal alkaline phosphatase. A microheterogeneous series of glycoproteins.
FEBS Lett. 1970 Mar 16;7(1):86-89 PMID: 11947438
Nuclear magnetic resonance spectroscopic studies of procaine hydrochloride and tetracaine hydrochloride at lipid-water interfaces.
Biochim Biophys Acta. 1969;183(3):466-75 PMID: 5822819
Mechanism of change in permeability in living membranes.
Nature. 1960 Dec 31;188:1209-10 PMID: 13750675
Secretion of alkaline phosphatase subunits by spheroplasts of Escherichia coli.
J Bacteriol. 1968 Sep;96(3):727-33 PMID: 4979102
Effect of phenethyl alcohol on induction of alkaline phosphatase in Escherichia coli.
Biochem Biophys Res Commun. 1970 Oct 9;41(1):244-50 PMID: 4918014

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.131.2.431-437.1977

Published

1977-08-00

Pages

431-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC235448

Subset

External Links

PubMed source DOI: 10.1128/jb.131.2.431-437.1977 PMC Full Text View journal details

Analysis Services

Induction of alkaline phosphatase in Escherichia coli: effect of procaine hydrochloride.

Contact

WeChat Official Account