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PMID: 3284745 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing.

The EMBO journal ·Vol. 7 ·No. 2 ·1988-02-00 ·Pages 473-83

Herbert CJ, Labouesse M, Dujardin G, Slonimski PP

Abstract

We have cloned and sequenced the NAM2 gene of Saccharomyces douglasii, which is a homologue of the NAM2 gene of Saccharomyces cerevisiae. The wild-type S.douglasii gene possesses the suppressor functions of the mutant S. cerevisiae NAM2-1 allele, being able to cure a mitochondrial b14 maturase deficiency. By sequence comparisons and direct measurements we have demonstrated that the NAM2 genes encode mitochondrial leucyl tRNA synthetases (EC 6.1.1.4.). Using a derivative of the NAM2 gene, where the expression of the gene is under the control of the UAS GAL10, we have shown that the processing of the pre-mRNA from the two mosaic genes oxi3 and cob-box is impaired when transcription of the gene is repressed. These results lead us to conclude that the mitochondrial leucyl tRNA synthetase is involved in protein synthesis and mRNA splicing. Sequence comparisons show that the mitochondrial and Escherichia coli leucyl tRNA synthetases are highly homologous; however, significant features which may be important for the splicing functions of the mitochondrial enzymes are absent from the bacterial enzyme.

MeSH Terms
Amino Acid Sequence Amino Acyl-tRNA Synthetases/genetics Base Sequence Cloning, Molecular DNA, Mitochondrial/genetics Escherichia coli/enzymology,genetics Genes Genes, Fungal Leucine-tRNA Ligase/genetics,metabolism Mitochondria/enzymology Molecular Sequence Data RNA Splicing RNA, Messenger/genetics Saccharomyces/enzymology,genetics Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid
Chemicals
DNA, Mitochondrial RNA, Messenger Amino Acyl-tRNA Synthetases Leucine-tRNA Ligase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Herbert C J
Centre de Génétique Moléculaire du CNRS, Laboratoire propre associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.
Labouesse M
Dujardin G
Slonimski P P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-02-00
Pages
473-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454344
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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