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PMID: 3283147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular targeting of isoproteins in muscle cytoarchitecture.

The Journal of cell biology ·Vol. 106 ·No. 4 ·1988-04-00 ·Pages 1161-70

Schäfer BW, Perriard JC

Abstract

Part of the muscle creatine kinase (MM-CK) in skeletal muscle of chicken is localized in the M-band of myofibrils, while chicken heart cells containing myofibrils and BB-CK, but not expressing MM-CK, do not show this association. The specificity of the MM-CK interaction was tested using cultured chicken heart cells as "living test tubes" by microinjection of in vitro generated MM-CK and hybrid M-CK/B-CK mRNA with SP6 RNA polymerase. The resulting translation products were detected in injected cells with isoprotein-specific antibodies. M-CK molecules and translation products of chimeric cDNA molecules containing the head half of the B-CK and the tail half of the M-CK coding regions were localized in the M-band of the myofibrils. The tail, but not the head portion of M-CK is essential for the association of M-CK with the M-band of myofibrils. We conclude that gross biochemical properties do not always coincide with a molecule's specific functions like the participation in cell cytoarchitecture which may depend on molecular targeting even within the same cellular compartment.

MeSH Terms
Amino Acid Sequence Animals Chick Embryo Connectin Creatine Kinase/analysis,genetics Fluorescent Antibody Technique Isoenzymes/analysis Microinjections Muscle Proteins/analysis Myocardium/enzymology Myofibrils/enzymology Protein Biosynthesis RNA, Messenger/genetics
Chemicals
Connectin Isoenzymes Muscle Proteins RNA, Messenger Creatine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schäfer B W
Institute for Cell Biology, Swiss Federal Institute of Technology, Zurich.
Perriard J C
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47 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.4.1161
Published
1988-04-00
Pages
1161-70
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115002
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.106.4.1161 PMC Full Text View journal details
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