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PMID: 3280947 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Function of cloned T4 recombination genes, uvsX and uvsY, in cells of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 211 ·No. 2 ·1988-02-00 ·Pages 350-6

Minagawa T, Fujisawa H, Yonesaki T, Ryo Y

Abstract

Genes uvsX and uvsY of bacteriophage T4 both control genetic recombination and repair of damaged DNA, and their mutant phenotypes bear a striking resemblance to each other. It has been shown recently that the uvsX gene product is analogous to the recA gene product of Escherichia coli (Yonesaki et al. 1985; Yonesaki and Minagawa 1985; Formosa and Alberts 1986), but the function of the uvsY gene is unknown. To obtain further insight into the function of these genes we introduced plasmidborne copies of the two genes separately or together into E. coli. The uvsX gene rendered recA- cells more resistant to UV and raised the recombination frequency of lambda phage and E. coli, but hampered induction of the lambda prophage and the SOS function of E. coli. The uvsY gene had no detectable function when introduced alone into E. coli but significantly enhanced the function of the uvsX gene when the two plasmid-borne genes were introduced together.

MeSH Terms
Cloning, Molecular DNA Damage DNA Repair Escherichia coli/genetics Genes, Viral Genotype Mutation Plasmids Rec A Recombinases/genetics Recombination, Genetic T-Phages/genetics
Chemicals
Rec A Recombinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Minagawa T
Department of Botany, Faculty of Science, Kyoto University, Japan.
Fujisawa H
Yonesaki T
Ryo Y
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330615
Published
1988-02-00
Pages
350-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330615
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