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PMID: 3277188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

In situ hybridization of nucleus basalis neurons shows increased beta-amyloid mRNA in Alzheimer disease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1227-31

Cohen ML, Golde TE, Usiak MF, Younkin LH, Younkin SG

Abstract

To determine which cells within the brain produce beta-amyloid mRNA and to assess expression of the beta-amyloid gene in Alzheimer disease, we analyzed brain tissue from Alzheimer and control patients by in situ hybridization. Our results demonstrate that beta-amyloid mRNA is produced by neurons in the nucleus basalis of Meynert and cerebral cortex and that nucleus basalis perikarya from Alzheimer patients consistently hybridize more beta-amyloid probe than those from controls. These observations support the hypothesis that increased expression of the beta-amyloid gene plays an important role in the deposition of amyloid in the brains of patients with Alzheimer disease.

MeSH Terms

Alzheimer Disease/metabolism Amyloid/biosynthesis Amyloid beta-Peptides Basal Ganglia/analysis Cerebral Cortex/analysis Humans Neurons/analysis Nucleic Acid Hybridization RNA, Messenger/analysis

Chemicals

Amyloid Amyloid beta-Peptides RNA, Messenger

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Cohen M L

Division of Neuropathology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106.

Golde T E

Usiak M F

Younkin L H

Younkin S G

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.4.1227

Published

1988-02-00

Pages

1227-31

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC279740

Subset

Grants

NIA NIH HHS · AG-06656 · United States

External Links

PubMed source DOI: 10.1073/pnas.85.4.1227 PMC Full Text View journal details

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In situ hybridization of nucleus basalis neurons shows increased beta-amyloid mRNA in Alzheimer disease.

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