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PMID: 3276710 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level.

The Journal of cell biology ·Vol. 106 ·No. 1 ·1988-01-00 ·Pages 141-9

Khawaja S, Gundersen GG, Bulinski JC

Abstract

Interphase cultured monkey kidney (TC-7) cells contain distinct subsets of cellular microtubules (MTs) enriched in posttranslationally detyrosinated (Glu) or tyrosinated (Tyr) alpha tubulin (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779-789). To determine the relative stability of these subsets of MTs, we subjected TC-7 cells to treatments that slowly depolymerized MTs. We found Glu MTs to be more resistant than Tyr MTs to depolymerization by nocodazole in living cells, and to depolymerization by dilution in detergent-permeabilized cell models. However, in cold-treated cells, Glu and Tyr MTs did not differ significantly in their stability. Digestion of permeabilized cell models with pancreatic carboxypeptidase A, to generate Glu MTs from endogenous Tyr MTs, did not significantly alter the resistance of the endogenous Tyr MTs toward dilution-induced depolymerization. Furthermore, in human fibroblasts that contained no distinct Glu MTs, we observed a population of nocodazole-resistant MTs. These data suggest that Glu MTs possess enhanced stability against end-mediated depolymerization, yet detyrosination alone appears to be insufficient to confer this enhanced stability.

MeSH Terms
Animals Benzimidazoles/pharmacology Chlorocebus aethiops Cold Temperature Cytoskeleton/physiology Fluorescent Antibody Technique Glutamates Microtubules/drug effects,physiology Nocodazole Polymers Protein Binding Solubility Tubulin/physiology Tyrosine/physiology
Chemicals
Benzimidazoles Glutamates Polymers Tubulin Tyrosine Nocodazole
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Khawaja S
Department of Biology, University of California, Los Angeles 90024.
Gundersen G G
Bulinski J C
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39 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.1.141
Published
1988-01-00
Pages
141-9
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114950
Subset
IM
Grants
NCI NIH HHS · CA39755 · United States
External Links
PubMed source DOI: 10.1083/jcb.106.1.141 PMC Full Text View journal details
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