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PMID: 327087 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.

Journal of virology ·Vol. 22 ·No. 3 ·1977-06-00 ·Pages 619-25

Mukai R, Hayashi M

Abstract

An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isolated from 20S material was open circular replicative form (RF), DNA in 50S material was replicative-form DNA with an extended single-stranded viral DNA that ranged up to one genome in length, and DNA in 90S material consisted of circular and linear single-stranded viral DNA of full genome length and single-stranded viral DNA shorter than full genome length. Pulse and pulse-chase experiments indicated that 90S material derived from 50S material.

MeSH Terms

Cell-Free System Coliphages/metabolism DNA, Circular/biosynthesis DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli Models, Biological Thymine Nucleotides/metabolism

Chemicals

DNA, Circular DNA, Single-Stranded DNA, Viral Thymine Nucleotides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mukai R

Hayashi M

References (15)

15 references, click to expand

Functions of gene C and gene D products of bacteriophage phi X 174.
J Virol. 1977 Feb;21(2):506-15 PMID: 833936
Two proteins of gene A of psiX174.
Nat New Biol. 1973 Sep 5;245(140):6-8 PMID: 4516942
A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.
Biochim Biophys Acta. 1966 Jan 18;114(1):158-68 PMID: 4287053
Gene A of X174. 1. Isolation and identification of its products.
Virology. 1972 Nov;50(2):381-7 PMID: 4564522
Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids.
Biochemistry. 1971 Nov;10(23):4212-8 PMID: 4942455
DNA replication: the rolling circle model.
Cold Spring Harb Symp Quant Biol. 1968;33:473-84 PMID: 4891987
Stages in the replication of bacteriophage phi X174 DNA in vivo.
Cold Spring Harb Symp Quant Biol. 1968;33:443-7 PMID: 4891984
Bacteriophage phi-X174 and related viruses.
Prog Nucleic Acid Res Mol Biol. 1968;8:115-69 PMID: 4874231
The single-stranded DNA phages.
CRC Crit Rev Microbiol. 1975 Dec;4(2):161-223 PMID: 1106952
Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo.
J Virol. 1976 Aug;19(2):416-24 PMID: 957477
Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.
J Virol. 1976 Aug;19(2):409-15 PMID: 957476
The rolling circle for phiX DNA replication. II. Synthesis of single-stranded circles.
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1934-42 PMID: 5276153
The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.
J Mol Biol. 1969 Oct 28;45(2):237-63 PMID: 5367027
Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.
J Virol. 1969 Oct;4(4):400-7 PMID: 5823229
Mechanism of replication of phi-X-174 single stranded DNA.
Nature. 1968 Jul 27;219(5152):346-51 PMID: 5662147

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.22.3.619-625.1977

Published

1977-06-00

Pages

619-25

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515760

Subset

External Links

PubMed source DOI: 10.1128/JVI.22.3.619-625.1977 PMC Full Text View journal details

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In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.

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