Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.

The Journal of experimental medicine ·Vol. 167 ·No. 5 ·1988-05-01 ·Pages 1572-85

Lanier LL, Buck DW, Rhodes L, Ding A, Evans E, Barney C, Phillips JH

Abstract

IL-2 potentiates both growth and cytotoxic function of T lymphocytes and NK cells. Resting peripheral blood NK cells can respond directly to rIL-2, without requirement for accessory cells or cofactors, and enhanced cytotoxicity can be measured within a few hours after exposure to this lymphokine. In this study, we describe an activation antigen, Leu-23, that is rapidly induced and phosphorylated after IL-2 stimulation of NK cells and a subset of low buoyant density T lymphocytes. Previously, it has been uncertain whether all NK cells or only a subset are responsive to IL-2. Since within 18 h after exposure to IL-2, essentially all NK cells express Leu-23, these findings indicate that all peripheral blood NK cells are responsive to stimulation by IL-2. The Leu-23 antigen is a disulfide-bonded homodimer, composed of 24-kD protein subunits with two N-linked oligosaccharides. Appearance of this glycoprotein on NK cells is IL-2 dependent and closely parallels IL-2-induced cytotoxicity against NK-resistant solid tumor cell targets.

MeSH Terms

Antigens, CD Antigens, Differentiation, T-Lymphocyte Antigens, Surface/biosynthesis Cytotoxicity, Immunologic Gene Expression Regulation Humans Interleukin-2/pharmacology Killer Cells, Natural/drug effects,immunology,metabolism Lectins, C-Type Phosphorylation Protein Processing, Post-Translational Recombinant Proteins/pharmacology T-Lymphocytes/classification,drug effects,immunology

Chemicals

Antigens, CD Antigens, Differentiation, T-Lymphocyte Antigens, Surface CD69 antigen Interleukin-2 Lectins, C-Type Recombinant Proteins

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Lanier L L

Becton Dickinson Monoclonal Center, Inc., Mountain View, California 94043.

Buck D W

Rhodes L

Ding A

Evans E

Barney C

Phillips J H

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.167.5.1572

Published

1988-05-01

Pages

1572-85

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2188935

Subset

External Links

PubMed source DOI: 10.1084/jem.167.5.1572 PMC Full Text View journal details

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Interleukin 2 activation of natural killer cells rapidly induces the expression and phosphorylation of the Leu-23 activation antigen.

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