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PMID: 3242867 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Presence of a 16S rRNA pseudogene in the bi-molecular plastid genome of the primitive brown alga Pylaiella littoralis. Evolutionary implications.

Current genetics ·Vol. 14 ·No. 6 ·1988-12-00 ·Pages 599-608

Markowicz Y, Loiseaux-de Goër S, Mache R

Abstract

The plastid genome of the brown alga Pylaiella littoralis (L.) Kjellm. is composed of two different circular DNA molecules: the largest carries two rrn operons, and the smallest, only one copy of both 16S and 23S rDNAs. 16S rDNA copies located on both molecules have been cloned and their nucleotide sequences determined: they are 65% homologous to one another. The expression of these genes was assayed by hybridizing in vivo labelled P. littoralis rRNAs to both clones, and specific oligonucleotides to total RNA from P. littoralis. Results indicate that the 16S rDNA copy located on the small molecule is a pseudogene. Comparisons of the functional gene with other 16S rRNA genes shows that chloroplasts from green plants emerged earlier from the cyanobacterial lineage than Euglena gracilis and Pylaiella littoralis plastids.

MeSH Terms
Base Sequence Biological Evolution Cloning, Molecular DNA Probes DNA, Ribosomal/genetics Deoxyribonuclease EcoRI Eukaryota/genetics Gene Expression Regulation Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization Phaeophyta/genetics Phylogeny Pseudogenes RNA, Ribosomal/genetics RNA, Ribosomal, 16S/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA Probes DNA, Ribosomal RNA, Ribosomal RNA, Ribosomal, 16S Deoxyribonuclease EcoRI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Markowicz Y
Laboratoire de Biologie Moléculaire Végétale, CNRS UA 1178, Université J. Fourier, Grenoble, France.
Loiseaux-de Goër S
Mache R
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00434086
Published
1988-12-00
Pages
599-608
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00434086 View journal details
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