Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.

Journal of clinical microbiology ·Vol. 26 ·No. 12 ·1988-12-00 ·Pages 2667-8

Hector RF, Zimmer BL, Pappagianis D

Abstract

A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37 degrees C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.

MeSH Terms

Antifungal Agents/pharmacology Coccidioides/drug effects,physiology Microbial Sensitivity Tests/methods Spores, Bacterial/drug effects,physiology

Chemicals

Antifungal Agents

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hector R F

Cutter Biologicals, Berkeley, California 94701.

Zimmer B L

Pappagianis D

References (15)

15 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.26.12.2667-2668.1988

Published

1988-12-00

Pages

2667-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC266970

Subset

External Links

PubMed source DOI: 10.1128/jcm.26.12.2667-2668.1988 PMC Full Text View journal details

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Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.

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