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PMID: 32290493 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

High Expression of the Sda Synthase B4GALNT2 Associates with Good Prognosis and Attenuates Stemness in Colon Cancer.

Cells ·Vol. 9 ·No. 4 ·2020-00-11

Pucci M, Gomes Ferreira I, Orlandani M, Malagolini N, Ferracin M, Dall'Olio F

Abstract

The carbohydrate antigen Sda and its biosynthetic enzyme B4GALNT2 are highly expressed in normal colonic mucosa but are down-regulated to a variable degree in colon cancer tissues. Here, we investigated the clinical and biological importance of B4GALNT2 in colon cancer. Correlations of B4GALNT2 mRNA with clinical data were obtained from The Cancer Genome Atlas (TCGA) database; the phenotypic and transcriptomic changes induced by B4GALNT2 were studied in LS174T cells transfected with B4GALNT2 cDNA. TCGA data indicate that patients with high B4GALNT2 expression in cancer tissues display longer survival than non-expressers. In LS174T cells, expression of B4GALNT2 did not affect the ability to heal a scratch wound or to form colonies in standard growth conditions but markedly reduced the growth in soft agar, the tridimensional (3D) growth as spheroids, and the number of cancer stem cells, indicating a specific effect of B4GALNT2 on the growth in poor adherence and stemness. On the transcriptome, B4GALNT2 induced the down-regulation of the stemness-associated gene SOX2 and modulated gene expression towards an attenuation of the cancer phenotype. The level of B4GALNT2 can be proposed as a marker to identify higher- and lower-risk colorectal cancer patients.

Keywords
gene expression glycosylation glycosyltransferases microarray analysis stemness sugar antigens
MeSH Terms
Colonic Neoplasms/genetics,metabolism Female Gene Expression Glycosylation Humans Male Microarray Analysis/methods N-Acetylgalactosaminyltransferases/metabolism Oligosaccharides/metabolism Prognosis Transfection
Chemicals
Oligosaccharides Sd(a) determinant N-Acetylgalactosaminyltransferases beta-1,4-N-acetyl-galactosaminyl transferase 2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pucci Michela
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
Gomes Ferreira Inês
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
Orlandani Martina
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
Malagolini Nadia
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
Ferracin Manuela ORCID
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
Dall'Olio Fabio ORCID
Department of Experimental, Diagnostic and Specialty Medicine (DIMES), General Pathology building, University of Bologna, Via San Giacomo 14, Via San Giacomo 14, 40126 Bologna, Italy.
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Article Info
Journal
Cells
Abbr.
Cells
ISSN
2073-4409
DOI
10.3390/cells9040948
Published
2020-00-11
Epub
2020-00-11
Language
English
Region
Switzerland
NLM ID
101600052
PMCID
PMC7226961
Subset
IM
External Links
PubMed source DOI: 10.3390/cells9040948 PMC Full Text View journal details
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