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PMID: 32284544 Published · ppublish English Journal Article

An RNA thermoswitch regulates daytime growth in Arabidopsis.

Nature plants ·Vol. 6 ·No. 5 ·2020-00-00 ·Pages 522-532

Chung BYW, Balcerowicz M, Di Antonio M, Jaeger KE, Geng F, Franaszek K, Marriott P, Brierley I, Firth AE, Wigge PA

Abstract

Temperature is a major environmental cue affecting plant growth and development. Plants often experience higher temperatures in the context of a 24 h day-night cycle, with temperatures peaking in the middle of the day. Here, we find that the transcript encoding the bHLH transcription factor PIF7 undergoes a direct increase in translation in response to warmer temperature. Diurnal expression of PIF7 transcript gates this response, allowing PIF7 protein to quickly accumulate in response to warm daytime temperature. Enhanced PIF7 protein levels directly activate the thermomorphogenesis pathway by inducing the transcription of key genes such as the auxin biosynthetic gene YUCCA8, and are necessary for thermomorphogenesis to occur under warm cycling daytime temperatures. The temperature-dependent translational enhancement of PIF7 messenger RNA is mediated by the formation of an RNA hairpin within its 5' untranslated region, which adopts an alternative conformation at higher temperature, leading to increased protein synthesis. We identified similar hairpin sequences that control translation in additional transcripts including WRKY22 and the key heat shock regulator HSFA2, suggesting that this is a conserved mechanism enabling plants to respond and adapt rapidly to high temperatures.

MeSH Terms
Arabidopsis/growth & development,physiology Arabidopsis Proteins/physiology Circadian Rhythm DNA-Binding Proteins/physiology Gene Expression Regulation, Plant RNA, Plant/physiology Temperature Transcription Factors/physiology
Chemicals
Arabidopsis Proteins DNA-Binding Proteins PIF7 protein, Arabidopsis RNA, Plant Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Chung Betty Y W ORCID
Department of Plant Sciences, University of Cambridge, Cambridge, UK. bcy23@cam.ac.uk. | Department of Pathology, University of Cambridge, Cambridge, UK. bcy23@cam.ac.uk.
Balcerowicz Martin ORCID
Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Di Antonio Marco ORCID
Department of Chemistry, Molecular Science Research Hub, Imperial College London, London, UK.
Jaeger Katja E
Sainsbury Laboratory, University of Cambridge, Cambridge, UK. | Leibniz-Institut für Gemüse- und Zierpflanzenbau, Großbeeren, Germany.
Geng Feng
Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Franaszek Krzysztof
Department of Pathology, University of Cambridge, Cambridge, UK.
Marriott Poppy
Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Brierley Ian
Department of Pathology, University of Cambridge, Cambridge, UK.
Firth Andrew E
Department of Pathology, University of Cambridge, Cambridge, UK.
Wigge Philip A ORCID
Department of Plant Sciences, University of Cambridge, Cambridge, UK. wigge@igzev.de. | Sainsbury Laboratory, University of Cambridge, Cambridge, UK. wigge@igzev.de. | Leibniz-Institut für Gemüse- und Zierpflanzenbau, Großbeeren, Germany. wigge@igzev.de. | Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany. wigge@igzev.de.
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Article Info
Journal
Nature plants
Abbr.
Nat Plants
ISSN
2055-0278
DOI
10.1038/s41477-020-0633-3
Email
bcy23@cam.ac.uk
wigge@igzev.de
Published
2020-00-00
Epub
2020-00-13
Pages
522-532
Language
English
Region
England
NLM ID
101651677
PMCID
PMC7231574
Subset
IM
Grants
Wellcome Trust · 096082 · United Kingdom
Wellcome Trust · 106207 · United Kingdom
Medical Research Council · MR/R021821/1 · United Kingdom
Biotechnology and Biological Sciences Research Council · BB/R011605/1 · United Kingdom
Corrections
CommentIn
PMID: 32284550
External Links
PubMed source DOI: 10.1038/s41477-020-0633-3 PMC Full Text View journal details
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