ppGpp cycle in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 150 ·No. 3 ·1977-02-15 ·Pages 249-55

Kari C, Török I, Travers A

Abstract

Kinetics of accumulation and degradation of ppGpp and pppGpp were analysed in spoT+ and spoT strains of Escherichia coli. The experimental data in this paper indicate that on degradation ppGpp is not converted to pppGpp but instead is converted to GDP which is in turn phosphorylated to GTP. In addition the data are consistent with the idea the pppGpp is a direct precursor of ppGpp. We propose that ppGpp is metabolised according to the following pathway: GTP-pppGpp--ppGpp--GDP--GTP, which we call the ppGpp cycle. Coupled with the observations in spot strains we assume that ppGpp blocks its own synthesis by inhibiting the synthesis of pppGpp but not the interconversion of the two nucleotides.

MeSH Terms

Amino Acids/metabolism Escherichia coli/metabolism Guanine Nucleotides/metabolism Histidine/metabolism Kinetics Mutation Serine/metabolism Species Specificity

Chemicals

Amino Acids Guanine Nucleotides Serine Histidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kari C

Török I

Travers A

References (21)

21 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268123

Published

1977-02-15

Pages

249-55

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268123

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ppGpp cycle in Escherichia coli.

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