Home LiteratureArticle Details
PMID: 3214436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions.

The Biochemical journal ·Vol. 255 ·No. 3 ·1988-11-01 ·Pages 999-1005

Enrich C, Bachs O, Evans WH

Abstract

The distribution of calmodulin-binding polypeptides in various rat liver subcellular fractions was investigated. Plasma-membrane, endosome, Golgi and lysosome fractions were prepared by established procedures. The calmodulin-binding polypeptides present in the subcellular fractions were identified by using an overlay technique after transfer from gels to nitrocellulose sheets. Distinctive populations of calmodulin-binding polypeptides were present in all the fractions examined except lysosomes. A major 115 kDa calmodulin-binding polypeptide of pI 4.3 was located to the endosome subfractions, and it emerges as a candidate endosome-specific protein. Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Major calmodulin-binding polypeptides of 140 and 240 kDa and minor polypeptides of 40-60 kDa were present in plasma membranes. The distribution of calmodulin in the various endosome and plasma-membrane fractions was also analysed, and the results indicated that the amounts were high compared with those in the cytosol.

MeSH Terms
Animals Calmodulin-Binding Proteins/metabolism Cell Membrane/metabolism Cytosol/metabolism Electrophoresis, Polyacrylamide Gel Endocytosis Intracellular Membranes/metabolism Liver/metabolism Peptides/analysis,metabolism Rats Subcellular Fractions/metabolism
Chemicals
Calmodulin-Binding Proteins Peptides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Enrich C
Laboratory of Protein Structure, National Institute for Medical Research, London, U.K.
Bachs O
Evans W H
References (44)
44 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Calmodulin and calmodulin-binding proteins in liver cell nuclei.
    J Biol Chem. 1987 Aug 5;262(22):10786-90 PMID: 3611090
  3. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  4. Functional polarity of the rat hepatocyte surface membrane. Isolation and characterization of plasma-membrane subfractions from the blood-sinusoidal, bile-Canalicular and contiguous surfaces of the hepatocyte.
    Biochem J. 1975 Feb;146(2):375-88 PMID: 125584
  5. Isolation of rat liver lysosomes by isopycnic centrifugation in a metrizamide gradient.
    J Cell Biol. 1978 Aug;78(2):349-68 PMID: 211139
  6. Calmodulin plays a pivotal role in cellular regulation.
    Science. 1980 Jan 4;207(4426):19-27 PMID: 6243188
  7. Phase separation of integral membrane proteins in Triton X-114 solution.
    J Biol Chem. 1981 Feb 25;256(4):1604-7 PMID: 6257680
  8. Fodrin: axonally transported polypeptides associated with the internal periphery of many cells.
    J Cell Biol. 1981 Sep;90(3):631-42 PMID: 6169732
  9. A major calmodulin-binding protein common to various vertebrate tissues.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3780-4 PMID: 6954520
  10. Calmodulin and the regulation of the actin-myosin interaction in smooth muscle and nonmuscle cells.
    Cell. 1982 Sep;30(2):349-50 PMID: 6128074
  11. Widespread occurrence of avian spectrin in nonerythroid cells.
    Cell. 1982 Jul;29(3):821-33 PMID: 6758951
  12. Purification and characterization of rabbit liver calmodulin-dependent glycogen synthase kinase.
    J Biol Chem. 1983 Feb 25;258(4):2376-82 PMID: 6296143
  13. Galactose-specific endocytosis in rat liver. Biochemical and morphological characterization of a low-density compartment isolated from hepatocytes.
    Eur J Biochem. 1983 Mar 1;131(1):105-12 PMID: 6832135
  14. Inositol trisphosphate and diacylglycerol: two interacting second messengers.
    Annu Rev Biochem. 1987;56:159-93 PMID: 3304132
  15. Isolation of an endocytic compartment from A431 cells using a density modification procedure employing a receptor-specific monoclonal antibody complexed with colloidal gold.
    J Cell Sci. 1987 May;87 ( Pt 4):495-506 PMID: 2888775
  16. Isolation and characterization of three endosomal fractions from the liver of estradiol-treated rats.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6785-9 PMID: 3477810
  17. Isolation and characterization of a Mr = 110,000 glycoprotein localized to the hepatocyte bile canaliculus.
    J Biol Chem. 1987 Oct 25;262(30):14753-9 PMID: 3312195
  18. The preparative isolation of endosome fractions: a review.
    Cell Biochem Funct. 1987 Oct;5(4):235-43 PMID: 2890445
  19. "Calciosome," a cytoplasmic organelle: the inositol 1,4,5-trisphosphate-sensitive Ca2+ store of nonmuscle cells?
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1091-5 PMID: 3257572
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Receptor-mediated endocytosis in rat liver: purification and enzymic characterization of low density organelles involved in uptake of galactose-exposing proteins.
    J Cell Biol. 1984 Mar;98(3):877-84 PMID: 6699090
  22. Inhibition by calmodulin of calcium/phospholipid-dependent protein phosphorylation.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3622-5 PMID: 6233611
  23. Identification of calmodulin-binding proteins in chicken embryo fibroblasts.
    J Cell Biol. 1984 Aug;99(2):550-7 PMID: 6086671
  24. Antibodies against lysosomal membranes reveal a 100,000-mol-wt protein that cross-reacts with purified H+,K+ ATPase from gastric mucosa.
    J Cell Biol. 1984 Oct;99(4 Pt 1):1511-26 PMID: 6207183
  25. Endocytosis of beta-adrenergic ligands by rat liver. Comparison of beta-adrenergic receptor and adenylate cyclase distribution in endosome and plasma-membrane fractions.
    Biochem J. 1984 Sep 15;222(3):749-54 PMID: 6091623
  26. In vivo uptake of insulin into hepatic Golgi fractions: application of the diaminobenzidine-shift protocol.
    Biochem Biophys Res Commun. 1984 Sep 28;123(3):1144-8 PMID: 6435623
  27. Stimulation of the purified erythrocyte Ca2+-ATPase by tryptic fragments of calmodulin.
    J Biol Chem. 1984 Dec 25;259(24):15172-7 PMID: 6239867
  28. Hepatic endosome fractions contain an ATP-driven proton pump.
    Biochem J. 1985 Jan 1;225(1):51-8 PMID: 2983664
  29. The association of calmodulin with subcellular fractions isolated from rat liver.
    Biochem Biophys Res Commun. 1985 Feb 28;127(1):358-65 PMID: 2983716
  30. Preparation of low-density "endosome" and "endosome"-depleted Golgi fractions from rat liver.
    Methods Enzymol. 1985;109:246-57 PMID: 2859513
  31. Effect of trifluoperazine on DNA synthesis during liver regeneration.
    Cell Tissue Kinet. 1985 Sep;18(5):475-81 PMID: 4028107
  32. Desmocalmin: a calmodulin-binding high molecular weight protein isolated from desmosomes.
    J Cell Biol. 1985 Dec;101(6):2070-80 PMID: 2415534
  33. Receptor-mediated endocytosis.
    Biochem J. 1985 Nov 15;232(1):1-14 PMID: 2867759
  34. Subfractionation of hepatic endosomes in Nycodenz gradients and by free-flow electrophoresis. Separation of ligand-transporting and receptor-enriched membranes.
    Biochem J. 1985 Nov 15;232(1):25-32 PMID: 2867760
  35. Phospholipid, cholesterol, polypeptide and glycoprotein composition of hepatic endosome subfractions.
    Biochem J. 1985 Nov 15;232(1):33-6 PMID: 2867761
  36. Receptor-mediated endocytosis of asialoglycoproteins by rat hepatocytes: receptor-positive and receptor-negative endosomes.
    J Cell Biol. 1986 Mar;102(3):932-42 PMID: 3512582
  37. The interaction of calmodulin with rat liver plasma membrane.
    Biochem Biophys Res Commun. 1986 Feb 26;135(1):323-9 PMID: 3954776
  38. Identification of a transformation-sensitive 110-kDa plasma membrane glycoprotein of rat hepatocytes.
    Eur J Cell Biol. 1986 Jan;39(2):417-23 PMID: 3007150
  39. Purification and characterization of a sea urchin egg Ca2+-calmodulin-dependent kinase with myosin light chain phosphorylating activity.
    J Biol Chem. 1986 Apr 25;261(12):5389-95 PMID: 2937787
  40. Separation of two populations of endocytic vesicles involved in receptor-ligand sorting in rat hepatocytes.
    J Biol Chem. 1986 Jun 5;261(16):7445-54 PMID: 3011784
  41. Characterization of endocytic compartments using the horseradish peroxidase-diaminobenzidine density shift technique.
    J Cell Biol. 1987 Jan;104(1):77-85 PMID: 2878931
  42. Rapid analytical and preparative isolation of functional endosomes by free flow electrophoresis.
    J Cell Biol. 1987 Apr;104(4):875-86 PMID: 3031085
  43. Identification of a fodrin-like protein in rat liver basolateral membranes.
    Biochem Biophys Res Commun. 1987 Jun 30;145(3):1033-7 PMID: 3606592
  44. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2550999
Published
1988-11-01
Pages
999-1005
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135340
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2550999 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2