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PMID: 3208741 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two guanosine binding sites exist in group I self-splicing IVS RNAs.

The EMBO journal ·Vol. 7 ·No. 11 ·1988-11-00 ·Pages 3531-7

Kay PS, Menzel P, Inoue T

Abstract

A shortened form of the self-splicing rRNA intervening sequence (IVS) of Tetrahymena thermophila can catalyze a transesterification reaction, termed G-exchange, between a monomeric guanosine derivative such as GTP and the substrate GpN (where N is A, C, G or U). The reaction is specific to the two guanosines involved, providing evidence that two guanosine binding sites exist in this group I IVS RNA. One binding site accommodates a guanosine which initiates self-splicing and the other recognizes the guanosine preceding the 3' splice site. Previously, only one guanosine binding site was thought to be involved in the mechanism of self-splicing. Based on the two functionally distinguishable guanosine binding sites, a new model is proposed to explain how the two independent transesterification reactions required for self-splicing might proceed in a concerted manner.

MeSH Terms
Animals Binding Sites Guanosine/metabolism Guanosine Triphosphate/metabolism Introns Kinetics Nucleotides/metabolism RNA Splicing RNA, Ribosomal/metabolism Tetrahymena/genetics
Chemicals
Nucleotides RNA, Ribosomal Guanosine Guanosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kay P S
Salk Institute for Biological Studies, San Diego, CA 92138.
Menzel P
Inoue T
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32 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-11-00
Pages
3531-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454854
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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