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PMID: 3196314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Erythrocyte protein 4.1 associates with tubulin.

The Biochemical journal ·Vol. 255 ·No. 1 ·1988-10-01 ·Pages 217-21

Correas I, Avila J

Abstract

Protein 4.1 binds to tubulin, as determined by sedimentation and immunoelectron-microscopy analyses, at a molar ratio similar to that described for brain microtubule-associated proteins. The binding site appears to be located at the C-terminal region of tubulin. Experiments performed in situ by adding exogenous protein 4.1 to permeabilized 3T3 cells show that this protein binds to microtubule and nuclear components.

MeSH Terms
Animals Cytoskeletal Proteins Electrophoresis, Polyacrylamide Gel Erythrocyte Membrane/metabolism Immunoblotting Membrane Proteins/metabolism,ultrastructure Microscopy, Electron Microtubules/metabolism,ultrastructure Neuropeptides Protein Binding Tubulin/metabolism,ultrastructure
Chemicals
Cytoskeletal Proteins Membrane Proteins Neuropeptides Tubulin erythrocyte membrane band 4.1 protein erythrocyte membrane protein band 4.1-like 1
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Correas I
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Avila J
References (25)
25 references, click to expand
  1. A circular dichroism study of the interaction of chlorpromazine with mouse brain tubulin.
    Biochemistry. 1978 Oct 31;17(22):4735-9 PMID: 728382
  2. Preferential binding of hog brain microtubule-associated proteins to mouse satellite versus bulk DNA preparations.
    Nature. 1978 Jun 1;273(5661):403-5 PMID: 661954
  3. In vitro formation of a complex between cytoskeletal proteins of the human erythrocyte.
    Nature. 1979 Aug 30;280(5725):811-4 PMID: 471052
  4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  5. Associations of erythrocyte membrane proteins. Binding of purified bands 2.1 and 4.1 to spectrin.
    J Biol Chem. 1980 Jul 25;255(14):7034-9 PMID: 6771281
  6. A genetic defect in the binding of protein 4.1 to spectrin in a kindred with hereditary spherocytosis.
    N Engl J Med. 1982 Nov 25;307(22):1367-74 PMID: 6215583
  7. Glycophorin is linked by band 4.1 protein to the human erythrocyte membrane skeleton.
    Nature. 1984 Feb 16-22;307(5952):655-8 PMID: 6694756
  8. Monoclonal antibody against microtubule associated protein-1 produces immunofluorescent spots in the nucleus and centrosome of cultured mammalian cells.
    Cell Struct Funct. 1983 Sep;8(3):245-54 PMID: 6368013
  9. A structural model of human erythrocyte protein 4.1.
    J Biol Chem. 1984 Apr 10;259(7):4603-8 PMID: 6707022
  10. A variant of erythrocyte membrane skeletal protein band 4.1 associated with hereditary elliptocytosis.
    Blood. 1984 Nov;64(5):1006-15 PMID: 6487803
  11. Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction.
    Biochemistry. 1984 Sep 25;23(20):4675-81 PMID: 6388633
  12. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  13. Interactions between protein 4.1 and band 3. An alternative binding site for an element of the membrane skeleton.
    J Biol Chem. 1985 Mar 25;260(6):3676-83 PMID: 3972843
  14. The membrane skeleton of human erythrocytes and its implications for more complex cells.
    Annu Rev Biochem. 1985;54:273-304 PMID: 3161450
  15. Localization of the tubulin binding site for tau protein.
    Eur J Biochem. 1985 Dec 16;153(3):595-600 PMID: 3935441
  16. Identification of the functional site of erythrocyte protein 4.1 involved in spectrin-actin associations.
    J Biol Chem. 1986 Mar 5;261(7):3310-5 PMID: 3949771
  17. The localization of tau proteins on the microtubule surface.
    Eur J Cell Biol. 1986 Apr;40(2):233-7 PMID: 3086098
  18. Structure of the spectrin-actin binding site of erythrocyte protein 4.1.
    J Biol Chem. 1986 Oct 5;261(28):13362-6 PMID: 3531202
  19. Common and distinct tubulin binding sites for microtubule-associated proteins.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7162-6 PMID: 3463956
  20. Stabilizing infrastructure of cell membranes.
    Annu Rev Cell Biol. 1985;1:531-61 PMID: 3916322
  21. Analysis of the red cell membrane in a family with hereditary elliptocytosis--total or partial of protein 4.1.
    Hum Genet. 1981;59(1):68-71 PMID: 10819025
  22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  23. Microtubule assembly in the absence of added nucleotides.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 PMID: 4514990
  24. A protein factor essential for microtubule assembly.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1858-62 PMID: 1057175
  25. Differences in surface morphology of microtubules reconstituted from pure brain tubulin using two different microtubule-associated proteins: the high molecular weight MAP 2 proteins and tau proteins.
    Eur J Cell Biol. 1979 Jun;19(2):175-83 PMID: 467462
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1988-10-01
Pages
217-21
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135212
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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