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PMID: 3193361 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production.

Journal of basic microbiology ·Vol. 28 ·No. 4 ·1988-00-00 ·Pages 211-20

Chen XJ, Wésolowski-Louvel M, Tanguy-Rougeau C, Bianchi MM, Fabiani L, Saliola M, Falcone C, Frontali L, Fukuhara H

Abstract

A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 microns/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.

MeSH Terms

Cloning, Molecular DNA, Fungal/genetics Fungal Proteins/biosynthesis,genetics Genes, Fungal Genetic Vectors Killer Factors, Yeast Kluyveromyces/genetics,metabolism Mycotoxins/biosynthesis,genetics Plasmids Promoter Regions, Genetic Saccharomyces cerevisiae Proteins Saccharomycetales/genetics Transformation, Genetic

Chemicals

DNA, Fungal Fungal Proteins KHR1 protein, S cerevisiae Killer Factors, Yeast Mycotoxins Saccharomyces cerevisiae Proteins

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Chen X J

Institut Curie, Centre Universitaire Paris-Sud, Orsay, France.

Wésolowski-Louvel M

Tanguy-Rougeau C

Bianchi M M

Fabiani L

Saliola M

Falcone C

Frontali L

Fukuhara H

Article Info

Journal

Journal of basic microbiology

Abbr.

J Basic Microbiol

ISSN

0233-111X

DOI

10.1002/jobm.3620280402

Published

1988-00-00

Pages

211-20

Language

English

Region

Germany

NLM ID

8503885

Subset

External Links

PubMed source DOI: 10.1002/jobm.3620280402 View journal details

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A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production.

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