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PMID: 31875359 Published · ppublish English Journal Article

LncRNA MIR4435-2HG-mediated upregulation of TGF-β1 promotes migration and proliferation of nonsmall cell lung cancer cells.

Environmental toxicology ·Vol. 35 ·No. 5 ·2020-05-00 ·Pages 582-590

Yang M, He X, Huang X, Wang J, He Y, Wei L

Abstract

The roles of long noncoding RNAs (lncRNAs) have been shown to play critical roles in tumor progression. Here, it was identified that lncRNA MIR4435-2HG was highly expressed in lung cancer tissues, especially in nonsmall cell lung cancer (NSCLC). A consistent result was obtained in lung cancer cells. Functional experiments showed that knockdown of MIR4435-2HG reduced the proliferation and migration ability of NSCLC cells. Transcriptome-sequencing analysis indicated that TGF-β signaling was mostly enriched in NSCLC cells with MIR4435-2HG knockdown. Furthermore, MIR4435-2HG was identified as an miRNA sponge for TGF-β1 and thus activated TGF-β signaling. Additionally, re-activation of TGF-β1 rescued MIR4435-2HG knockdown-mediated inhibition on the progression of NSCLC cells. Therefore, this work indicates a novel MIR4435-2HG/TGF-β1 axis responsible for NSCLC cell progression.

Keywords
MIR4435-2HG TGF-β1 lncRNA miRNA nonsmall cell lung cancer
MeSH Terms
Adult Aged Carcinoma, Non-Small-Cell Lung/blood,genetics,pathology Cell Line, Tumor Cell Movement/genetics Cell Proliferation/genetics Disease Progression Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Humans Lung Neoplasms/blood,genetics,pathology Male MicroRNAs/blood,genetics Middle Aged RNA, Long Noncoding/blood,genetics Transcriptional Activation Transforming Growth Factor beta1/blood,genetics Up-Regulation
Chemicals
MIR4435-2HG long non-coding RNA, human MicroRNAs RNA, Long Noncoding TGFB1 protein, human Transforming Growth Factor beta1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yang Mei
Department of Respiratory and Critical Care, Yunnan Second People's Hospital, Kunming City, Yunnan Province, China.
He Xiaohua
Department of Respiratory and Critical Care, Yunnan Second People's Hospital, Kunming City, Yunnan Province, China.
Huang Xiaoxian
Department of Respiratory and Critical Care, Yunnan Second People's Hospital, Kunming City, Yunnan Province, China.
Wang Jiyang
Department of Cardiovascular Surgery, Yunnan Second People's Hospital, Kunming City, Yunnan Province, China.
He Yuxin
Keck School of Medicine, University of Southern California, Los Angeles, California.
Wei Li ORCID
Department of Respiratory and Critical Care, Yunnan Second People's Hospital, Kunming City, Yunnan Province, China.
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Article Info
Journal
Environmental toxicology
Abbr.
Environ Toxicol
ISSN
1522-7278
DOI
10.1002/tox.22893
Published
2020-05-00
Epub
2019-00-25
Pages
582-590
Language
English
Region
United States
NLM ID
100885357
Subset
IM
External Links
PubMed source DOI: 10.1002/tox.22893 View journal details
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