Home LiteratureArticle Details
PMID: 3185544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles.

Molecular and cellular biology ·Vol. 8 ·No. 10 ·1988-10-00 ·Pages 4134-42

Bucher EA, Maisonpierre PC, Konieczny SF, Emerson CP

Abstract

We compared the developmental regulation of the three troponin genes that encode the proteins of the Ca2+ regulatory complex in striated muscles of the Japanese quail. Nuclear run-on transcription and RNA protection analyses showed that the fast skeletal troponin I, the fast skeletal troponin T, and the slow skeletal-cardiac troponin C genes were transcriptionally coactivated and that transcripts rapidly accumulated within 6 to 12 h after the initiation of myoblast differentiation. The fast-isoform mRNAs of troponin I and troponin T were coexpressed at similar levels in different skeletal muscles, whereas the slow-cardiac troponin C mRNA varied independently and was the only one of these genes expressed in embryonic and adult heart. We conclude that these troponin genes are transcriptionally coactivated during skeletal myoblast differentiation, indicating that their transcription is under precise temporal control. However, this troponin C gene is regulated independently is specialized striated muscles.

MeSH Terms
Animals Cell Differentiation Coturnix Gene Expression Regulation Heart/embryology,physiology Muscles/cytology,embryology,physiology Myocardium/cytology RNA, Messenger/genetics Restriction Mapping Transcription, Genetic Troponin/genetics
Chemicals
RNA, Messenger Troponin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bucher E A
Department of Biology, University of Virginia, Charlottesville 22901.
Maisonpierre P C
Konieczny S F
Emerson C P
References (54)
54 references, click to expand
  1. Tissue-specific and developmentally regulated expression of a chimeric actin-globin gene in transgenic mice.
    Mol Cell Biol. 1986 Jul;6(7):2624-31 PMID: 3023942
  2. Differentiation of fast and slow muscles in the cat hind limb.
    J Physiol. 1960 Feb;150:399-416 PMID: 13805873
  3. Gene regulation for higher cells: a theory.
    Science. 1969 Jul 25;165(3891):349-57 PMID: 5789433
  4. Diffusion-mediated control of myoblast fusion.
    Dev Biol. 1971 Sep;26(1):133-52 PMID: 4329509
  5. Activation of myosin synthesis in fusing and mononucleated myoblasts.
    J Mol Biol. 1975 Apr 25;93(4):431-47 PMID: 806694
  6. Amino-acid sequence of tropomyosin-binding component of rabbit skeletal muscle troponin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1902-6 PMID: 1064862
  7. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  8. Post-transcriptional regulation of ribosome accumulation during myoblast differentiation.
    Cell. 1977 Apr;10(4):587-96 PMID: 558828
  9. Comparison of amino acid sequence of troponin I from different striated muscles.
    Nature. 1978 Jan 5;271(5640):31-5 PMID: 146828
  10. Coordinate regulation of contractile protein synthesis during myoblast differentiation.
    Cell. 1978 Apr;13(4):599-611 PMID: 657269
  11. Skeletal myoblasts in culture.
    Methods Enzymol. 1979;58:511-27 PMID: 423788
  12. Distribution of polymorphic forms of troponin components and tropomyosin in skeletal muscle.
    Nature. 1979 Apr 19;278(5706):714-8 PMID: 372827
  13. Coordinate accumulation of contractile protein mRNAs during myoblast differentiation.
    Dev Biol. 1979 Mar;69(1):202-16 PMID: 446892
  14. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
  15. Polymorphic forms of troponin T and troponin C and their localization in striated muscle cell types.
    Exp Cell Res. 1979 Sep;122(2):339-50 PMID: 116859
  16. Troponin C from rabbit slow skeletal and cardiac muscle is the product of a single gene.
    Eur J Biochem. 1980 Jan;103(1):179-88 PMID: 7358047
  17. Changes in gene expression during myogenic differentiation. I. Regulation of messenger RNA sequences expressed during myotube formation.
    J Mol Biol. 1980 Jul 15;140(4):441-58 PMID: 7431395
  18. Types of troponin components during development of chicken skeletal muscle.
    Dev Biol. 1981 Feb;82(1):11-9 PMID: 7227630
  19. The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of corresponding mRNA sequences during myogenesis.
    Dev Biol. 1981 Sep;86(2):483-92 PMID: 7286410
  20. Gene expression of myofibrillar proteins in single muscle fibers of adult chicken: micro two dimensional gel electrophoretic analysis.
    J Biochem. 1981 Jun;89(6):1951-62 PMID: 7287667
  21. Differentiation of troponin in cardiac and skeletal muscles in chicken embryos as studied by immunofluorescence microscopy.
    J Cell Biol. 1981 Nov;91(2 Pt 1):497-504 PMID: 6171575
  22. cDNA clone analysis of six co-regulated mRNAs encoding skeletal muscle contractile proteins.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1553-7 PMID: 6951196
  23. DNAase I sensitivity of genes expressed during myogenesis.
    Nucleic Acids Res. 1982 May 25;10(10):3085-98 PMID: 6285287
  24. Fibre types in chicken skeletal muscles and their changes in muscular dystrophy.
    J Physiol. 1982 Oct;331:333-54 PMID: 7153905
  25. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  26. Troponin subunit stoichiometry and content in rabbit skeletal muscle and myofibrils.
    J Biol Chem. 1983 May 10;258(9):5770-4 PMID: 6853545
  27. Glyceraldehyde 3-phosphate dehydrogenase protein and mRNA are both differentially expressed in adult chickens but not chick embryos.
    Nucleic Acids Res. 1983 May 25;11(10):3301-15 PMID: 6687938
  28. Transcriptional and cell cycle-mediated regulation of myosin heavy chain gene expression during muscle cell differentiation.
    J Biol Chem. 1983 Sep 25;258(18):11063-73 PMID: 6193119
  29. Developmentally regulated expression of chimeric genes containing muscle actin DNA sequences in transfected myogenic cells.
    EMBO J. 1984 May;3(5):983-90 PMID: 6329749
  30. Isoform variants of troponin in skeletal and cardiac muscle cells cultured with and without nerves.
    Cell. 1983 May;33(1):297-304 PMID: 6380757
  31. Differentiation of fiber types in wing muscles during embryonic development: effect of neural tube removal.
    Dev Biol. 1984 Dec;106(2):457-68 PMID: 6238862
  32. A single troponin T gene regulated by different programs in cardiac and skeletal muscle development.
    Science. 1984 Nov 23;226(4677):979-82 PMID: 6095446
  33. Matching of muscle properties and motoneurone firing patterns during early stages of development.
    J Exp Biol. 1985 Mar;115:113-23 PMID: 4031759
  34. Generation of troponin T isoforms by alternative RNA splicing in avian skeletal muscle. Conserved and divergent features in birds and mammals.
    J Biol Chem. 1985 Nov 5;260(25):13699-703 PMID: 3840482
  35. Structure, evolution, and regulation of a fast skeletal muscle troponin I gene.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8080-4 PMID: 3865218
  36. Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5' end of the chicken alpha-skeletal actin gene.
    Nucleic Acids Res. 1986 Feb 25;14(4):1683-701 PMID: 3005979
  37. Differentiation, not determination, regulates muscle gene activation: transfection of troponin I genes into multipotential and muscle lineages of 10T1/2 cells.
    Mol Cell Biol. 1985 Sep;5(9):2423-32 PMID: 2426582
  38. Transcriptional regulation of nicotinic acetylcholine receptor genes during muscle development.
    J Biol Chem. 1986 Sep 5;261(25):11452-5 PMID: 3745150
  39. Upstream regions of the human cardiac actin gene that modulate its transcription in muscle cells: presence of an evolutionarily conserved repeated motif.
    Mol Cell Biol. 1986 Jun;6(6):2125-36 PMID: 3785189
  40. Closely related alpha-tropomyosin mRNAs in quail fibroblasts and skeletal muscle cells.
    J Biol Chem. 1987 Mar 15;262(8):3590-6 PMID: 3818657
  41. Thyroid hormone regulates expression of a transfected alpha-myosin heavy-chain fusion gene in fetal heart cells.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3122-6 PMID: 3472199
  42. Cloning and characterization of mammalian myosin regulatory light chain (RLC) cDNA: the RLC gene is expressed in smooth, sarcomeric, and nonmuscle tissues.
    J Cell Biol. 1987 Jun;104(6):1505-13 PMID: 3584239
  43. The cloning and the codon and amino acid sequence of the quail slow/cardiac troponin C cDNA.
    Methods Enzymol. 1987;139:326-37 PMID: 3587028
  44. Structural aspects of troponin-tropomyosin regulation of skeletal muscle contraction.
    Annu Rev Biophys Biophys Chem. 1987;16:535-59 PMID: 2954560
  45. Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation.
    Science. 1987 Sep 18;237(4821):1473-6 PMID: 3498214
  46. Duplicated CArG box domains have positive and mutually dependent regulatory roles in expression of the human alpha-cardiac actin gene.
    Mol Cell Biol. 1987 Aug;7(8):2803-13 PMID: 2823106
  47. Complex regulation of the muscle-specific contractile protein (troponin I) gene.
    Mol Cell Biol. 1987 Sep;7(9):3065-75 PMID: 3670306
  48. A novel hybrid alpha-tropomyosin in fibroblasts is produced by alternative splicing of transcripts from the skeletal muscle alpha-tropomyosin gene.
    J Biol Chem. 1987 Nov 25;262(33):15998-6010 PMID: 2445743
  49. Alternative splicing generates variants in important functional domains of human slow skeletal troponin T.
    J Biol Chem. 1987 Nov 25;262(33):16122-6 PMID: 2824479
  50. Developmentally regulated expression of a truncated myosin light-chain 1F/3F gene.
    Mol Cell Biol. 1987 Oct;7(10):3826-9 PMID: 3683399
  51. The muscle creatine kinase gene is regulated by multiple upstream elements, including a muscle-specific enhancer.
    Mol Cell Biol. 1988 Jan;8(1):62-70 PMID: 3336366
  52. Differential patterns of transcript accumulation during human myogenesis.
    Mol Cell Biol. 1987 Nov;7(11):4100-14 PMID: 3431550
  53. Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene.
    Mol Cell Biol. 1988 Feb;8(2):1006-9 PMID: 2451118
  54. Transcriptional regulation of the muscle creatine kinase gene and regulated expression in transfected mouse myoblasts.
    Mol Cell Biol. 1986 Aug;6(8):2855-64 PMID: 3785216
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.10.4134-4142.1988
Published
1988-10-00
Pages
4134-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365482
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.10.4134-4142.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2