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PMID: 3185510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Polarity of DNA entry in transformation of Streptococcus pneumoniae.

Molecular & general genetics : MGG ·Vol. 213 ·No. 2-3 ·1988-08-00 ·Pages 444-8

Méjean V, Claverys JP

Abstract

DNA transport in Streptococcus pneumoniae was studied using donor molecules labelled either at the 3' or at the 5' end, on one strand only. In contrast to 5' end label, 3' end label was not taken up by the cells indicating that entry is a polarized process. Our results together with those of previous studies are consistent with a model for entry in which double-stranded donor DNA is nicked on binding at the cell surface. Entry of a single strand then proceeds linearly from a newly formed 3' end to the extremity of the donor fragment.

MeSH Terms
Biological Transport, Active DNA, Bacterial/genetics,metabolism Models, Genetic Streptococcus pneumoniae/genetics,metabolism Transformation, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Méjean V
Centre de Recherche de Biochimie et de Génétique Cellulaires du C.N.R.S., Université Paul Sabatier, Toulouse, France.
Claverys J P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00339614
Published
1988-08-00
Pages
444-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00339614
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