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PMID: 3184176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence implicating protein kinase C in exocytosis from electropermeabilized bovine chromaffin cells.

The Journal of membrane biology ·Vol. 104 ·No. 1 ·1988-08-00 ·Pages 21-34

Knight DE, Sugden D, Baker PF

Abstract

The calcium sensitivity of exocytosis from electro-permeabilized chromaffin cells is increased by activators of protein kinase C, such as TPA and certain phorbol esters, diacylglycerols, and mezerein. A range of putative inhibitors of protein kinase C block both the phorbol ester-sensitive component of secretion and also the underlying insensitive component. These inhibitors are also shown to inhibit medulla protein kinase C activity in vitro. The extent of secretion is reduced when electro-permeabilized cells are exposed to Ca2+ levels much in excess of 50 microM. The onset of inhibition is faster than the relatively slow rate of Ca-dependent exocytosis and is insensitive to inhibitors of proteolysis. Adrenal medulla protein kinase C activity is also irreversibly inhibited by high Ca2+ concentrations. Both the secretory response and the protein kinase C activity in vitro have similar nucleotide and cation specificities. Although these data do not definitely establish an involvement of protein kinase C in exocytosis, none argue against it.

MeSH Terms
Animals Aspirin/pharmacology Binding, Competitive Calcium/pharmacology,physiology Catecholamines/metabolism Cations, Divalent/pharmacology Cattle Cell Membrane Permeability Chromaffin System/cytology,physiology Diglycerides/biosynthesis,pharmacology Electric Stimulation Exocytosis/drug effects In Vitro Techniques Indomethacin/pharmacology Inositol/pharmacology Kinetics Molecular Weight Phorbol Esters/pharmacology Protein Kinase C/antagonists & inhibitors,isolation & purification,physiology Substrate Specificity
Chemicals
Catecholamines Cations, Divalent Diglycerides Phorbol Esters Inositol Protein Kinase C Aspirin Calcium Indomethacin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Knight D E
MRC Secretory Mechanism Group, Division of Biomedical Sciences, Kings College, London, England.
Sugden D
Baker P F
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871899
Published
1988-08-00
Pages
21-34
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01871899 View journal details
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