Abstract
The 120-kDa lysosomal membrane glycoprotein (lgp120) is an acidic, heavily glycosylated membrane protein enriched in the lysosomal membrane. To determine the basis for its selective transport to and stability in lysosomes, we have investigated the structure of lgp120. By using an oligonucleotide probe corresponding to the amino terminus of rat lgp120, we isolated and characterized cDNA clones containing the entire coding region. The deduced amino acid sequence demonstrates that lgp120 contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment, and a short (11 amino acid) cytosolic tail. The sequence suggests a distinct domain organization, with two luminal glycosylated regions separated by a nonglycosylated proline-rich region. Proteolysis in detergent showed that the protein was not intrinsically resistant to exogenous or endogenous proteases. The N-linked oligosaccharides on lgp120, tetraantennary structures with two lactosamine repeats on one of the branches, were not different from those of glycoproteins on the plasma membrane. lgp120 was similar in its domain organization and portions of its amino acid sequence to the avian 100-kDa lysosomal membrane protein LEP100 [Fambrough, D. M., Takeyasu, K., Lippincott-Schwartz, J., Siegel, N. R. & Somerville, D. (1988) J. Cell Biol. 106, 61-67], and to a distinct 110-kDa lysosomal membrane protein (lgp110) that colocalizes with lgp120. The similarities between lysosomal membrane glycoproteins from diverse species, coupled with the fact that at least two distinct lysosomal membrane glycoproteins are expressed in a single species, indicate the existence of a conserved family of glycoproteins enriched in the lysosomal membrane.
MeSH Terms
Amino Acid Sequence
Animals
Base Sequence
Biological Transport
Cell Membrane/analysis,metabolism
Cloning, Molecular
DNA/genetics
Lysosomes/analysis,metabolism,ultrastructure
Membrane Glycoproteins/analysis,genetics,metabolism
Molecular Sequence Data
Oligosaccharides/analysis
Protein Sorting Signals/genetics
Rats
Chemicals
Membrane Glycoproteins
Oligosaccharides
Protein Sorting Signals
DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Howe C L
Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510.
Granger B L
Hull M
Green S A
Gabel C A
Helenius A
Mellman I
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