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PMID: 31529521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The C4 Ppc promoters of many C4 grass species share a common regulatory mechanism for gene expression in the mesophyll cell.

The Plant journal : for cell and molecular biology ·Vol. 101 ·No. 1 ·2020-00-00 ·Pages 204-216

Gupta SD, Levey M, Schulze S, Karki S, Emmerling J, Streubel M, Gowik U, Paul Quick W, Westhoff P

Abstract

C4 photosynthetic plants have evolved from C3 ancestors and are characterized by differential expression of several hundred genes. Strict compartmentalization of key C4 enzymes either to mesophyll (M) or bundle sheath cells is considered a crucial step towards the evolution of C4 photosynthesis. In this study, we demonstrate that the 5'-flanking sequences of the C4 type phosphoenolpyruvate carboxylase (Ppc) gene from three C4 grass species could drive M-cell-specific expression of a reporter gene in rice. In addition to that, we identified about 450 bp (upstream of their transcription start site) of the analyzed C4 Ppc promoters contain all the essential regulatory elements for driving M-cell-specific expression in rice leaves. Importantly, four motifs of conserved nucleotide sequences (CNSs) were also determined, which are essential for the activity of the promoter. A putative interaction between the CNSs and an unknown upstream element(s) is required for driving M-cell-specific expression. This work identifies the evolutionary conservation of C4 Ppc regulatory mechanisms of multiple closely related C4 grass species.

Keywords
C4 photosynthesis conserved nucleotide sequence mesophyll cells phosphoenolpyruvate carboxylase (Ppc) gene
MeSH Terms
Gene Expression Regulation, Plant/genetics,physiology Mesophyll Cells/metabolism Phosphoenolpyruvate Carboxylase/metabolism Photosynthesis/genetics,physiology Plants, Genetically Modified/genetics,metabolism Promoter Regions, Genetic/genetics
Chemicals
Phosphoenolpyruvate Carboxylase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Gupta Shipan Das
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany. | Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakahli, 3814, Bangladesh.
Levey Myles
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
Schulze Stefanie
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
Karki Shanta
International Rice Research Institute, Los Banos, Laguna, 4031, Philippines. | National Citrus Development Program, Kirtipur, Kathmandu, Nepal.
Emmerling Jan
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
Streubel Monika
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany.
Gowik Udo
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany. | Department of Biology and Environmental Sciences, Carl Von Ossietzky University, D-26129, Oldenburg, Germany.
Paul Quick W
International Rice Research Institute, Los Banos, Laguna, 4031, Philippines. | Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK.
Westhoff Peter ORCID
Institute of Plant Molecular and Developmental Biology, Universitätsstrasse 1, Heinrich-Heine-University, 40225, Düsseldorf, Germany. | Cluster of Excellence on Plant Sciences from Complex Traits towards Synthetic Modules, 40225 Duesseldorf and, 50923, Cologne, Germany.
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Article Info
Journal
The Plant journal : for cell and molecular biology
Abbr.
Plant J
ISSN
1365-313X
DOI
10.1111/tpj.14532
Published
2020-00-00
Epub
2019-00-16
Pages
204-216
Language
English
Region
England
NLM ID
9207397
Subset
IM
Grants
Bill & Melinda Gates Foundation · United States
Databases
GENBANK
AF495586, MH675618, MH675617, MH675616, MH675615
External Links
PubMed source DOI: 10.1111/tpj.14532 View journal details
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