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PMID: 3148853 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of two chloramphenicol acetyltransferase genes from Clostridium butyricum and their expression in Escherichia coli and Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 214 ·No. 2 ·1988-10-00 ·Pages 328-32

Dubbert W, Luczak H, Staudenbauer WL

Abstract

Two non-homologous chloramphenicol (Cm) acetyltransferase (CAT) genes, designated catA and catB, were cloned from Clostridium butyricum type strains and characterized by restriction mapping. Both genes are efficiently expressed in Escherichia coli and Bacillus subtilis. In contrast to analogous genes from staphylococci and bacilli, gene expression is not dependent on induction by Cm. The genes are considered as chromosomal, since no association with endogenous plasmids was detectable. Southern hybridization revealed a homology between catA and the staphylococcal Cm resistance plasmid, pC194. The subunit size of the clostridial CAT enzymes expressed in E. coli was determined as 22.5 kDa (catA) and 24 kDa (catB), respectively. The C. butyricum cat genes provide potentially useful selection markers for the construction of cloning vectors from cryptic clostridial plasmids.

MeSH Terms
Bacillus subtilis/genetics Chloramphenicol O-Acetyltransferase/genetics Cloning, Molecular Clostridium/enzymology,genetics Escherichia coli/genetics Genes Genes, Bacterial Plasmids Restriction Mapping
Chemicals
Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dubbert W
Institute for Microbiology, Technical University Munich, Federal Republic of Germany.
Luczak H
Staudenbauer W L
References (33)
33 references, click to expand
  1. Purification and characterization of chloramphenicol acetyltransferase from Flavobacterium CB60.
    J Gen Microbiol. 1987 Aug;133(8):2115-22 PMID: 3327915
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Chloramphenicol acetyltransferase: enzymology and molecular biology.
    CRC Crit Rev Biochem. 1983;14(1):1-46 PMID: 6340955
  4. Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringens.
    J Bacteriol. 1987 Apr;169(4):1579-84 PMID: 2881919
  5. Chloramphenicol acetyltransferase gene of staphylococcal plasmid pC221. Nucleotide sequence analysis and expression studies.
    FEBS Lett. 1985 Jan 1;179(1):101-6 PMID: 3855295
  6. Cloning of a chloramphenicol acetyltransferase gene of Streptomyces acrimycini and its expression in Streptomyces and Escherichia coli.
    Gene. 1985;38(1-3):1-8 PMID: 3905512
  7. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
    Gene. 1981 Oct;15(1):81-93 PMID: 6271633
  8. A novel cloning vector for the direct selection of recombinant DNA in E. coli.
    Gene. 1982 Sep;19(2):231-4 PMID: 6293932
  9. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
    Gene. 1980 May;9(3-4):287-305 PMID: 6248430
  10. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  11. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  12. Deletion of C-terminal amino acid codons of PhiX174 gene E: effect on its lysis inducing properties.
    Nucleic Acids Res. 1985 Jun 11;13(11):4143-53 PMID: 2989789
  13. Bacterial resistance to chloramphenicol.
    Br Med Bull. 1984 Jan;40(1):36-41 PMID: 6398727
  14. Chloramphenicol acetylation in Streptomyces.
    J Gen Microbiol. 1976 May;94(1):159-66 PMID: 932687
  15. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  16. Characterization and transferability of Clostridium perfringens plasmids.
    Plasmid. 1977 Nov;1(1):52-66 PMID: 220651
  17. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.
    Methods Enzymol. 1975;43:737-55 PMID: 1094240
  18. The effects of deletions in the leader sequence of cat-86, a chloramphenicol-resistance gene isolated from Bacillus pumilus.
    Gene. 1987;54(2-3):267-73 PMID: 3477516
  19. Chloramphenicol-inducible gene expression in Bacillus subtilis.
    Gene. 1983 Oct;24(2-3):171-7 PMID: 6416927
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Plasmid detection and isolation in strains of Clostridium acetobutylicum and related species.
    Mol Gen Genet. 1983;189(1):178-80 PMID: 6574306
  22. Chloramphenicol induces translation of the mRNA for a chloramphenicol-resistance gene in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):3939-43 PMID: 3086871
  23. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  24. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
    Anal Biochem. 1977 May 1;79(1-2):544-52 PMID: 68686
  25. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  26. Constitutive variants of the pC194 cat gene exhibit DNA alterations in the vicinity of the ribosome binding site sequence.
    Gene. 1984 May;28(2):171-6 PMID: 6588016
  27. Nucleotide sequence of a Bacillus pumilus gene specifying chloramphenicol acetyltransferase.
    Gene. 1983 Oct;24(2-3):163-9 PMID: 6315534
  28. Identification of restriction fragments from two cryptic Clostridium butyricum plasmids that promote the establishment of a replication-defective plasmid in Bacillus subtilis.
    J Gen Microbiol. 1985 Aug;131(8):2097-105 PMID: 2997368
  29. Dependence of expression of an inducible Staphylococcus aureus cat gene on the translation of its leader sequence.
    Mol Gen Genet. 1987 May;207(2-3):486-91 PMID: 3112518
  30. Genetic characterization of recombination-deficient mutants of Bacillus subtilis.
    J Bacteriol. 1974 Feb;117(2):488-93 PMID: 4272775
  31. Affinity and hydrophobic chromatography of three variants of chloramphenicol acetyltransferases specified by R factors in Escherichia coli.
    FEBS Lett. 1976 Mar 1;62(3):266-71 PMID: 776682
  32. Characterization and comparison of chloramphenicol acetyltransferase variants.
    Eur J Biochem. 1979 Oct 15;100(2):609-18 PMID: 116849
  33. Regulation of the inducible chloramphenicol acetyltransferase gene of the Staphylococcus aureus plasmid pUB112.
    EMBO J. 1985 Sep;4(9):2295-300 PMID: 3865770
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337731
Published
1988-10-00
Pages
328-32
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337731
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