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题目:: Analysis of cytoplasmic factors in developmental cleavage of mouse embryo.
作者:: Suzuki(S),Komatsu(S),Kitai(H),Endo(Y),Iizuka(R),Fukasawa(T)
状态:: 发布时间1989-02-23 , 更新时间 2013-11-21
期刊:: Cell Differ
摘要:: One-cell embryos from certain mouse strains were found incapable of developing beyond the 2-cell stage in vitro (2-cell block), but a microinjection of EDTA effectively overcame this block. When 2-cell arrested embryos were fused with embryos that had developed to the late 2-cell stage in vivo, the fusants developed beyond the 2-cell stage. Microinjection of cytoplasm of in vivo 2-cell embryos into 1-cell embryos also obviated the 2-cell block. Analyses of 35S-labeled embryos by 2-dimensional polyacrylamide gel electrophoresis indicated changes in synthetic protein patterns possibly related to this block.
语言:: eng
DOI:

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