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PMID: 3141175 Published · ppublish English Comparative Study Journal Article

Comparison of different antigen preparations in an evaluation of the immune response to Campylobacter pylori.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology ·Vol. 7 ·No. 4 ·1988-08-00 ·Pages 570-5

Hirschl AM, Pletschette M, Hirschl MH, Berger J, Stanek G, Rotter ML

Abstract

This study presents a novel approach to the analysis of protein antigens of Campylobacter pylori for use in serology. Protein fractions of this bacterium were resolved in polyacrylamide gel electrophoresis, eluted from gel strips in an electric field and used for coating of microtiter plates in an ELISA-type assay run with a small set of sera from both infected and non-infected patients. Reactivity and discriminative power of the different fractionated antigens (1-9) and crude antigen preparations (A-C) were compared. Better discrimination was achieved between positive and negative sera with high molecular weight fractionated preparations (antigens 8 and 9) than with low molecular weight fractions. Among the crude antigen preparations, antigens A (sonicated whole cells) and C (ultracentrifugated sonicate) seem to have a better discriminative power than antigen B (acid glycin extract).

MeSH Terms
Antibodies, Bacterial/analysis,biosynthesis Antigens, Bacterial/analysis,immunology Campylobacter/immunology Campylobacter Infections/diagnosis,immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans
Chemicals
Antibodies, Bacterial Antigens, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hirschl A M
Hygiene-Institute, University of Vienna, Austria.
Pletschette M
Hirschl M H
Berger J
Stanek G
Rotter M L
References (27)
27 references, click to expand
  1. Seroepidemiology of Campylobacter pyloridis.
    N Z Med J. 1986 Sep 10;99(809):657-9 PMID: 3463897
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Enzyme-linked immunosorbent assay for Campylobacter pyloridis: correlation with presence of C. pyloridis in the gastric mucosa.
    J Infect Dis. 1987 Mar;155(3):488-94 PMID: 3805774
  4. Campylobacter pyloridis and gastritis.
    J Infect Dis. 1986 Apr;153(4):650-7 PMID: 3512736
  5. Detection of Campylobacter pyloridis in patients with antrum gastritis and peptic ulcers by culture, complement fixation test, and immunoblot.
    J Clin Microbiol. 1986 Nov;24(5):716-20 PMID: 3533982
  6. Campylobacter pyloridis, gastritis, and peptic ulceration.
    J Clin Pathol. 1986 Apr;39(4):353-65 PMID: 3517070
  7. Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni.
    J Gen Microbiol. 1987 Jan;133(1):163-70 PMID: 3309141
  8. Antibody to the gastric campylobacter-like organism ("Campylobacter pyloridis")--clinical correlations and distribution in the normal population.
    J Med Microbiol. 1986 Aug;22(1):57-62 PMID: 3735391
  9. Immunoblot confirmation of immune response to Campylobacter pyloridis in patients with duodenal ulcers.
    Med J Aust. 1986 Aug 4-18;145(3-4):133-5 PMID: 3526100
  10. Campylobacter pyloridis-associated gastritis.
    J Infect. 1986 Sep;13(2):107-13 PMID: 3531349
  11. Systemic and local antibody responses to gastric Campylobacter pyloridis in non-ulcer dyspepsia.
    Gut. 1986 Jun;27(6):642-7 PMID: 3721286
  12. A simple and rapid method for the isolation of peptides from sodium dodecyl sulfate-containing polyacrylamide gels.
    Anal Biochem. 1982 Mar 15;121(1):215-7 PMID: 7091681
  13. Pyloric Campylobacter infection and gastroduodenal disease.
    Med J Aust. 1985 Apr 15;142(8):439-44 PMID: 3982346
  14. Antibody response of patients against a 120 kDa surface protein of Campylobacter pylori.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Apr;268(2):271-6 PMID: 3394453
  15. Conservation and diversity of Campylobacter pyloridis major antigens.
    Infect Immun. 1987 May;55(5):1256-63 PMID: 3552997
  16. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration.
    Lancet. 1984 Jun 16;1(8390):1311-5 PMID: 6145023
  17. Campylobacter pyloridis--a new factor in peptic ulcer disease?
    Gut. 1986 Jun;27(6):635-41 PMID: 3721285
  18. The efficacy of antimicrobial treatment in Campylobacter pylori-associated gastritis and duodenal ulcer.
    Scand J Gastroenterol Suppl. 1988;142:76-81 PMID: 3166537
  19. Polyacrylamide gel electrophoresis of spiral bacteria from the gastric antrum.
    Lancet. 1984 Jun 16;1(8390):1349-50 PMID: 6145041
  20. Campylobacter pylori detected noninvasively by the 13C-urea breath test.
    Lancet. 1987 May 23;1(8543):1174-7 PMID: 2883491
  21. Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH.
    Am J Gastroenterol. 1987 Mar;82(3):192-9 PMID: 3826027
  22. Attempt to fulfil Koch's postulates for pyloric Campylobacter.
    Med J Aust. 1985 Apr 15;142(8):436-9 PMID: 3982345
  23. [Significance of campylobacter-like bacteria (CLO) in diseases of the stomach and duodenum. Facts, hypotheses, clinical perspectives].
    Med Klin (Munich). 1987 May 8;82(10):367-72 PMID: 3600538
  24. 14C-urea breath analysis, a non-invasive test for Campylobacter pylori in the stomach.
    Lancet. 1987 Jun 13;1(8546):1367-8 PMID: 2884468
  25. Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease.
    Gastroenterology. 1987 Aug;93(2):371-83 PMID: 3297911
  26. Frequency of occurrence of Campylobacter pylori and analysis of the systemic and local immune response.
    Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Oct;266(3-4):526-42 PMID: 3439387
  27. Clinical importance of Campylobacter pyloridis and associated serum IgG and IgA antibody responses in patients undergoing upper gastrointestinal endoscopy.
    J Clin Pathol. 1986 Feb;39(2):215-9 PMID: 3950046
Article Info
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Abbr.
Eur J Clin Microbiol Infect Dis
ISSN
0934-9723
DOI
10.1007/BF01962618
Published
1988-08-00
Pages
570-5
Language
English
Region
Germany
NLM ID
8804297
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01962618 View journal details
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